Team:Tuebingen/Notebook/Protocols/LB agar

From 2014.igem.org

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<h3>LB agar</h3>
<h3>LB agar</h3>
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<h4>Reagents</h4>
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<table border="0">
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  <colgroup>
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    <col width="100">
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    <col width="300">
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  </colgroup>
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  <tr>
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    <td style="text-align: center">10 g</td>
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    <td>Bacto-tryptone</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">5 g</td>
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    <td>yeast extract</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">10 g</td>
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    <td>NaCl</td>
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  </tr>
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</table>
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<ol>
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  <li> Add the regents to 800ml H2O</li>
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  <li> Adjust pH to 7.5 with NaOH</li>
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  <li> Add 15g agar</li>
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  <li> Melt agar into solution in the microwave</li>
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  <li> Adjust volume to 1L with dH2O</li>
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  <li> Sterilize by autoclaving</li>
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</ol>
</div>
</div>

Revision as of 22:08, 14 October 2014


LB agar

Reagents

10 g Bacto-tryptone
5 g yeast extract
10 g NaCl
  1. Add the regents to 800ml H2O
  2. Adjust pH to 7.5 with NaOH
  3. Add 15g agar
  4. Melt agar into solution in the microwave
  5. Adjust volume to 1L with dH2O
  6. Sterilize by autoclaving

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