Team:Tuebingen/Notebook/Protocols/laemmli

From 2014.igem.org

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<h4>Procedure</h4>
<h4>Procedure</h4>
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<ol>
 
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  <li> Add the regents to 800ml H2O</li>
 
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  <li> Adjust pH to 7.5 with NaOH</li>
 
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  <li> Add 15g agar</li>
 
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  <li> Melt agar into solution in the microwave</li>
 
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  <li> Adjust volume to 1L with dH2O</li>
 
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  <li> Sterilize by autoclaving</li>
 
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</ol>
 

Revision as of 22:08, 14 October 2014


Laemmli buffer

Reagents

277.8 mM Tris-HCl, pH 6.8
4.4 % SDS
44.4% (w/v) glycerol
0.02 % bromophenol blue
10 % β-Mercaptoethanol

Procedure