Team:Tuebingen/Notebook/Protocols/laemmli

From 2014.igem.org

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<h4>Procedure</h4>
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<ol>
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  <li> Add the regents to 800ml H2O</li>
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  <li> Adjust pH to 7.5 with NaOH</li>
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  <li> Add 15g agar</li>
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  <li> Melt agar into solution in the microwave</li>
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  <li> Adjust volume to 1L with dH2O</li>
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  <li> Sterilize by autoclaving</li>
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</ol>

Revision as of 22:08, 14 October 2014


Laemmli buffer

Reagents

277.8 mM Tris-HCl, pH 6.8
4.4 % SDS
44.4% (w/v) glycerol
0.02 % bromophenol blue
10 % β-Mercaptoethanol

Procedure

  1. Add the regents to 800ml H2O
  2. Adjust pH to 7.5 with NaOH
  3. Add 15g agar
  4. Melt agar into solution in the microwave
  5. Adjust volume to 1L with dH2O
  6. Sterilize by autoclaving

Retrieved from "http://2014.igem.org/Team:Tuebingen/Notebook/Protocols/laemmli"