Team:Wageningen UR/overview/parts

From 2014.igem.org

(Difference between revisions)
Line 17: Line 17:
<th>Category</th>
<th>Category</th>
<th>BioBricks used</th>
<th>BioBricks used</th>
-
<th>Backbone</th>
+
 
-
<th>Size (bp)</th>
+
</tr>
-
<th>Sequenced yes/no</th>
+
<tr>
-
<th>documented yes/no</th>
+
<td>BBa_K1493000</td>
-
<th>shipped yes/no</th>
+
<td>FA regulation system</td>
 +
<td>Promoter only functional in presence of fusaric acid</td>
 +
<td>Regulator</td>
 +
<td>-</td>
</tr>
</tr>
<tr>
<tr>
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<td>Promoter only functional in presence of fusaric acid</td>
<td>Promoter only functional in presence of fusaric acid</td>
<td>Regulator</td>
<td>Regulator</td>
-
<td>-</td>
+
<td>-</td>
-
<td>pSB1C3</td>
+
</tr>
-
<td>1117</td>
+
<tr>
-
<td>yes</td>
+
<td>BBa_K1493003</td>
-
<td>yes</td>
+
<td>FA regulation system</td>
-
<td>yes</td>
+
<td>Promoter only functional in presence of fusaric acid, produces GFP when active</td>
 +
<td>Regulator</td>
 +
<td>Bba_I13504</td>
</tr>
</tr>
<tr>
<tr>
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<td>Transcription factor</td>
<td>Transcription factor</td>
<td>Bba_B0034</td>
<td>Bba_B0034</td>
-
<td>pSB1C3</td>
+
</tr>
-
<td></td>
+
<tr>
-
<td>yes</td>
+
<td>BBa_K1493300</td>
-
<td>yes</td>
+
<td>methionine-lyase</td>
-
<td>no</td>
+
<td>Converts L-methionine to methanethiol ammonium and 2-oxobutanoate</td>
 +
<td>enzyme</td>
 +
<td>-</td>
 +
</tr>
</tr>
<tr>
<tr>
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<td>Composite</td>
<td>Composite</td>
<td>BBa_R0010</td>
<td>BBa_R0010</td>
-
<td>pSB1C3</td>
+
</tr>
-
<td></td>
+
<tr>
-
<td>yes</td>
+
<td>BBa_K1493602</td>
-
<td>yes</td>
+
<td>LacI + Kid</td>
-
<td>no</td>
+
<td>Broad range toxin</td>
 +
<td>Composite</td>
 +
<td>BBa_J04500</td>
</tr>
</tr>
<tr>
<tr>
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<td>Reporter</td>
<td>Reporter</td>
<td>BBa_K914003 BBa_I13504</td>
<td>BBa_K914003 BBa_I13504</td>
-
<td>pSB1C3</td>
+
</tr>
-
<td></td>
+
<tr>
-
<td>yes</td>
+
<td>BBa_K1493502</td>
-
<td>no</td>
+
<td>pcI/lac+GFP</td>
-
<td>no</td>
+
<td>GFP controlled by pcI/lac</td>
 +
<td>Reporter</td>
 +
<td>BBa_K909012 BBa_I13504</td>
</tr>
</tr>
<tr>
<tr>
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<td>Reporter</td>
<td>Reporter</td>
<td>BBa_K909013 BBa_I13504</td>
<td>BBa_K909013 BBa_I13504</td>
-
<td>pSB1C3</td>
+
</tr>
-
<td></td>
+
<tr>
-
<td>yes</td>
+
<td>BBa_K1493504</td>
-
<td>no</td>
+
<td>pTet+GFP</td>
-
<td>no</td>
+
<td>GFP controlled by pTet</td>
 +
<td>Reporter</td>
 +
<td>BBa_R0040 BBa_I13504</td>
</tr>
</tr>
<tr>
<tr>
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<td>Composite</td>
<td>Composite</td>
<td>BBa_K914003 BBa_P0451</td>
<td>BBa_K914003 BBa_P0451</td>
-
<td>pSB1C3</td>
+
</tr>
-
<td></td>
+
<tr>
-
<td>yes</td>
+
<td>BBa_K1493520</td>
-
<td>no</td>
+
<td>pRHA+LacI</td>
-
<td>no</td>
+
<td>Repressor protein LacI controlled by pRha</td>
 +
<td>Composite</td>
 +
<td>BBa_K914003 BBa_P0412</td>
</tr>
</tr>
<tr>
<tr>
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<td>Composite</td>
<td>Composite</td>
<td>BBa_K914003 BBa_P0440</td>
<td>BBa_K914003 BBa_P0440</td>
-
<td>pSB1C3</td>
+
</tr>
-
<td></td>
+
<tr>
-
<td>yes</td>
+
<td>BBa_K1493540</td>
-
<td>no</td>
+
<td>pRHA+cI?+LacI</td>
-
<td>no</td>
+
<td>Repressor proteins cI lambda and LacI controlled by pRha</td>
 +
<td>Composite</td>
 +
<td>BBa_K914003 BBa_S0103 BBa_P0412</td>
</tr>
</tr>
<tr>
<tr>
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<td>Composite</td>
<td>Composite</td>
<td>BBa_K914003 BBa_I13504</td>
<td>BBa_K914003 BBa_I13504</td>
-
<td>pSB1C3</td>
 
-
<td></td>
 
-
<td>no</td>
 
-
<td>no</td>
 
-
<td>no</td>
 
</tr>
</tr>
<tr>
<tr>
-
+
<td>BBa_K1493560</td>
 +
<td>pRHA+cI?+pcI/tet+GFP</td>
 +
<td>Kill Switch Input/output</td>
 +
<td>Reporter</td>
 +
<td>BBa_K914003 BBa_P0451 BBa_K909013 BBa_I13504</td>
</tr>
</tr>
</table>
</table>

Revision as of 18:29, 14 October 2014

Wageningen UR iGEM 2014

 

 

 

Parts



    Part code Name Function Category BioBricks used
    BBa_K1493000 FA regulation system Promoter only functional in presence of fusaric acid Regulator -
    BBa_K1493002 FA regulation system Promoter only functional in presence of fusaric acid Regulator -
    BBa_K1493003 FA regulation system Promoter only functional in presence of fusaric acid, produces GFP when active Regulator Bba_I13504
    BBa_K1493200 PfrI Transcription factor involved in pyoverdine synethesis gene transcription Transcription factor Bba_B0034
    BBa_K1493300 methionine-lyase Converts L-methionine to methanethiol ammonium and 2-oxobutanoate enzyme -
    BBa_K1493601 LacI + Kis Kid antitoxin Composite BBa_R0010
    BBa_K1493602 LacI + Kid Broad range toxin Composite BBa_J04500
    BBa_K1493501 pRha+GFP GFP controlled by pRha Reporter BBa_K914003 BBa_I13504
    BBa_K1493502 pcI/lac+GFP GFP controlled by pcI/lac Reporter BBa_K909012 BBa_I13504
    BBa_K1493503 pcI/tet+GFP GFP controlled by pcI/tet Reporter BBa_K909013 BBa_I13504
    BBa_K1493504 pTet+GFP GFP controlled by pTet Reporter BBa_R0040 BBa_I13504
    BBa_K1493510 pRHA+cI? Repressor protein cI lambda controlled by pRha Composite BBa_K914003 BBa_P0451
    BBa_K1493520 pRHA+LacI Repressor protein LacI controlled by pRha Composite BBa_K914003 BBa_P0412
    BBa_K1493530 pRHA+TetR Repressor protein TetR controlled by pRha Composite BBa_K914003 BBa_P0440
    BBa_K1493540 pRHA+cI?+LacI Repressor proteins cI lambda and LacI controlled by pRha Composite BBa_K914003 BBa_S0103 BBa_P0412
    BBa_K1493550 pRHA+cI?+TetR Repressor proteins cI lambda and TetR controlled by pRha Composite BBa_K914003 BBa_I13504
    BBa_K1493560 pRHA+cI?+pcI/tet+GFP Kill Switch Input/output Reporter BBa_K914003 BBa_P0451 BBa_K909013 BBa_I13504

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