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From 2014.igem.org

Revision as of 18:14, 14 October 2014 (view source) Slysnake (Talk | contribs) ← Older edit		Revision as of 18:25, 14 October 2014 (view source) Slysnake (Talk | contribs) Newer edit →
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	<table width="70%" align="center">		<table width="70%" align="center">
-	</td>	+	<!--Our parts-->
		+	<tr><td><h3><font color="#558e2b"> Our parts </font></h3></td>
		+	<td></td>
	<tr> <td colspan="3"  height="10px"> </td></tr>		<tr> <td colspan="3"  height="10px"> </td></tr>
	<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>		<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
	<tr> <td colspan="3"  height="5px"> </td></tr>		<tr> <td colspan="3"  height="5px"> </td></tr>
-
	<!--Parts Submitted to the Registry  -->		<!--Parts Submitted to the Registry  -->
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	Synechocystis		Synechocystis
	is done by modifying the chromosome,		is done by modifying the chromosome,
-	rather than inserting plasmids. Synechocystis is na	+	rather than inserting plasmids. Synechocystis is naturally transformable, and will
-	turally transformable, and will	+	undergo homologous recombination between its chromosome and a plasmid
-	undergo homologous recombination between its chromo	+	containing a homologous region. This means that plasmids for insertion or
-	some and a plasmid	+	deletion need to have regions of ~500 to ~1000bp either side of the inserted
-	containing a homologous region. This means that pla	+	sequence, making modifications time consuming or costly depending on your
-	smids for insertion or	+	method of plasmid construction. We will therefore submit all our BioBricks for
-	deletion need to have regions of ~500 to ~1000bp ei	+	insertions and deletions to the registry. All of these will contain Kanamycin
-	ther side of the inserted	+
-	sequence, making modifications time consuming or co	+
-	stly depending on your	+
-	method of plasmid construction. We will therefore s	+
-	ubmit all our BioBricks for	+
-	insertions and deletions to the registry. All of th	+
-	ese will contain Kanamycin	+
	resistance for selecting transformants.		resistance for selecting transformants.
	<br />		<br />
	<br />		<br />
-	The mechanism for each of the BioBricks is the same	+	The mechanism for each of the BioBricks is the same. They will undergo
-	. They will undergo	+	recombination with the region that they share homology with. Knockouts will
-	recombination with the region that they share homol	+
-	ogy with. Knockouts will	+
	undergo recombination with the specified gene, repl		undergo recombination with the specified gene, repl
-	acing it will kanamycin	+	acing it will kanamycin resistance. Insertions will undergo recombination with a region of the
-	resistance. Insertions will undergo recombination w	+
-	ith a region of the	+
	chromosome that is not important for the metabolic		chromosome that is not important for the metabolic
-	conditions we are using, and	+	conditions we are using, and will insert the gene of interest and a kanamycin resistance gene.
-	will insert the gene of interest and a kanamycin re	+
-	sistance gene.	+
	<br />		<br />
	<br />		<br />
	We are creating 7 BioBricks that will be submitted		We are creating 7 BioBricks that will be submitted
	to registry, consisting of 4		to registry, consisting of 4
-	deletions and 3 insertions, in addition to improvin	+	deletions and 3 insertions, in addition to improving characterization of an
-	g characterization of an	+
	existing BioBrick. Background information on the ai		existing BioBrick. Background information on the ai
	m of these parts can be		m of these parts can be
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	<tr> <td colspan="3"  height="15px"> </td></tr>		<tr> <td colspan="3"  height="15px"> </td></tr>
-	<tr><td colspan="3" > <h3> Parts Table</h3></td></tr>	+	<tr><td><h3><font color="#558e2b"> Reading iGEM 2014 parts </font></h3></td>
-		+	<td></td>
-		+
-	<tr><td width="45%" colspan="3"  valign="top">	+
-	Any parts your team has created will appear in this table below:</td></tr>	+
	</table>		</table>

---

Revision as of 18:25, 14 October 2014

University of Reading
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Genetic modification in Synechocystis is done by modifying the chromosome, rather than inserting plasmids. Synechocystis is naturally transformable, and will undergo homologous recombination between its chromosome and a plasmid containing a homologous region. This means that plasmids for insertion or deletion need to have regions of ~500 to ~1000bp either side of the inserted sequence, making modifications time consuming or costly depending on your method of plasmid construction. We will therefore submit all our BioBricks for insertions and deletions to the registry. All of these will contain Kanamycin resistance for selecting transformants.

The mechanism for each of the BioBricks is the same. They will undergo recombination with the region that they share homology with. Knockouts will undergo recombination with the specified gene, repl acing it will kanamycin resistance. Insertions will undergo recombination with a region of the chromosome that is not important for the metabolic conditions we are using, and will insert the gene of interest and a kanamycin resistance gene.

We are creating 7 BioBricks that will be submitted to registry, consisting of 4 deletions and 3 insertions, in addition to improving characterization of an existing BioBrick. Background information on the ai m of these parts can be found in our “Project” section.

Our parts
Reading iGEM 2014 parts

<groupparts>iGEM013 Reading</groupparts>

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