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Team:Paris Saclay/Notebook/July/4
From 2014.igem.org
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=Friday 4th July= | =Friday 4th July= | ||
==Lab Work== | ==Lab Work== | ||
| - | === | + | ===-The chassis coli Odor free=== |
====Liquid cultures of MG1655 and MG1655Z1==== | ====Liquid cultures of MG1655 and MG1655Z1==== | ||
The transduction correctly worked. Here the results: | The transduction correctly worked. Here the results: | ||
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Liquid cultures at 37°C: | Liquid cultures at 37°C: | ||
| - | *2 colonies of MG1655 | + | *2 colonies of MG1655 from 100µl dilution |
| - | *1 colony of MG1655 | + | *1 colony of MG1655 from 50µl dilution |
In a tube, add 1 colony of MG1655Z1 in 5ml LB medium, leave at 37°C during the weekend. | In a tube, add 1 colony of MG1655Z1 in 5ml LB medium, leave at 37°C during the weekend. | ||
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===English Day=== | ===English Day=== | ||
To celebrate the 4th of July our team spoke in English all day. | To celebrate the 4th of July our team spoke in English all day. | ||
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| + | ==Photo of the Day== | ||
| + | [[File:Paris Saclay 4_july.jpg|600px|center]] | ||
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'''Members present:''' | '''Members present:''' | ||
*Instructors : Alice & Solenne. | *Instructors : Alice & Solenne. | ||
*Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain. | *Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain. | ||
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{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 15:35, 14 October 2014
Contents |
Friday 4th July
Lab Work
-The chassis coli Odor free
Liquid cultures of MG1655 and MG1655Z1
The transduction correctly worked. Here the results:
| Dilution for 100µl phage | MG1655 (colonies grown) | MG1655Z1 (colonies grown) |
|---|---|---|
|
100µl |
9 |
0 |
| 10µl | 2 | 2 |
| 50µl | 4 | 0 |
| 0µl | 0 | 0 |
Liquid cultures at 37°C:
- 2 colonies of MG1655 from 100µl dilution
- 1 colony of MG1655 from 50µl dilution
In a tube, add 1 colony of MG1655Z1 in 5ml LB medium, leave at 37°C during the weekend.
Wiki Progress
Wiki's notebook set up by Fabio.
Human Pratices
English Day
To celebrate the 4th of July our team spoke in English all day.
Photo of the Day
Members present:
- Instructors : Alice & Solenne.
- Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
