Team:Paris Saclay/Notebook/July/21

From 2014.igem.org

(Difference between revisions)
(→3 - Oligonucleotide's design)
m (→Monday 21st July)
 
(11 intermediate revisions not shown)
Line 1: Line 1:
 +
{{Team:Paris_Saclay/notebook_header}}
=Monday 21st July=
=Monday 21st July=
-
 
==Lab Work==
==Lab Work==
-
 
+
===The chassis coli Odor free===
-
===1 - Results: Transformation of CaCl<sub>2</sub> supercompetent cells===
+
====Results: Transformation of CaCl<sub>2</sub> supercompetent cells====
''by Romain''
''by Romain''
Line 9: Line 9:
from Bacterial Cultures transformed the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 18th July]
from Bacterial Cultures transformed the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 18th July]
-
Results: Nothing has grown.
+
'''Results:''' Nothing has grown.
-
===2 - Results: Transformation of DY330 via pJBEI6409===
+
====Results: Transformation of DY330 via pJBEI6409====
''by Sean''
''by Sean''
Line 40: Line 40:
|}
|}
-
'''Conclusion: The increase in colony number is proportional to the increase in volume used. The control yielded nothing. The results are coherent.'''
+
'''Conclusion:''' The increase in colony number is proportional to the increase in volume used. The control yielded nothing. The results are coherent.
-
===3 - Oligonucleotide's design===
+
===Lemon scent===
-
''by Romain''
+
-
 
+
-
For the banana scent, design of iPS91 and iPS92, the ilvE sequence to transformed the Leucin into α-cetoisocaproate (The first step).
+
-
===4 - Liquid Bacterial Culture===
+
====Liquid Bacterial Culture====
''by Marie, Romain & Sean''
''by Marie, Romain & Sean''
Line 54: Line 51:
*The new strains received
*The new strains received
-
===5 - Electrophoresis===
+
====Electrophoresis====
''by Fabio (process A) and Mathieu (process B and C)''
''by Fabio (process A) and Mathieu (process B and C)''
Line 65: Line 62:
'''''Process A'''''
'''''Process A'''''
-
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 J23119 Cl1]
+
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 BBa_J23119 Cl1]
-
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 J23119 Cl2]
+
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 BBa_J23119 Cl2]
-
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23106 Cl1]
+
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 BBa_J23106 Cl1]
-
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23100 Cl2]  
+
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 BBa_J23100 Cl2]  
#PCR 1
#PCR 1
#PCR 2
#PCR 2
Line 81: Line 78:
'''''Process B'''''
'''''Process B'''''
-
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23100 Cl1]
+
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 BBa_J23100 Cl1]
-
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23100 Cl2]
+
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 BBa_J23100 Cl2]
-
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23106 Cl1]
+
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 BBa_J23106 Cl1]
-
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23106 Cl2]
+
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 BBa_J23106 Cl2]
-
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23114 Cl1]
+
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 BBa_J23114 Cl1]
-
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 J23114 Cl2]
+
#[https://2014.igem.org/Team:Paris_Saclay/Notebook/July/16 BBa_J23114 Cl2]
'''''Process C'''''
'''''Process C'''''
Line 103: Line 100:
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
 +
 +
==Photo of the Day==
 +
[[File:Paris Saclay 21_july.jpg|400px|center]]
'''People there''':
'''People there''':
Line 108: Line 108:
* Students: Arnaud, Fabio, Juliette, Mathieu, Marie, Pierre, Romain, Sean and Terry.
* Students: Arnaud, Fabio, Juliette, Mathieu, Marie, Pierre, Romain, Sean and Terry.
-
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
+
{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 15:24, 14 October 2014

Contents

Monday 21st July

Lab Work

The chassis coli Odor free

Results: Transformation of CaCl2 supercompetent cells

by Romain

Strains transformed: MG1655, MG1655Z1. from Bacterial Cultures transformed the 18th July

Results: Nothing has grown.

Results: Transformation of DY330 via pJBEI6409

by Sean

Transformation performed on the 18th July

Number of colonies per dish (source of bacteria: electroporation cuvettes prepared on the 18th July)
Volume of plasmid used 50μl from cuvette 100μl remainder (850µl)
2μl of plasmid 0 4 30
4μl 3 6 45
Control 0 0 0

Conclusion: The increase in colony number is proportional to the increase in volume used. The control yielded nothing. The results are coherent.

Lemon scent

Liquid Bacterial Culture

by Marie, Romain & Sean

  • DY330 pJBEI6409 with 10µl Cm in 10ml LB (x2)
  • BT340 Cm and Amp
  • The new strains received

Electrophoresis

by Fabio (process A) and Mathieu (process B and C)

LU000069.jpg
LU000071.jpg
LU000070.jpg

We used 2µl of DNA of the following Biobricks in a 1% Agarose Gel. The PCRs came from Mathias' manipulation made the 18th July.

Process A

  1. BBa_J23119 Cl1
  2. BBa_J23119 Cl2
  3. BBa_J23106 Cl1
  4. BBa_J23100 Cl2
  5. PCR 1
  6. PCR 2
  7. PCR 3
  8. PCR 4
  9. PCR 5
  10. PCR 6
  11. PCR 7
  12. PCR 8
  13. PCR 9
  14. PCR 10

Process B

  1. BBa_J23100 Cl1
  2. BBa_J23100 Cl2
  3. BBa_J23106 Cl1
  4. BBa_J23106 Cl2
  5. BBa_J23114 Cl1
  6. BBa_J23114 Cl2

Process C

Pooling and purifying PCR 9 and 10 from process A.

  1. PCR purified products. IPS70 / IPS71 of pOsV230 (Apramycin)
  2. BT 340 (plasmid's flipase)

Results:

  • A: From 1 to 4: Success, DNAs have the expected size.
  • A: From 5 to 12: Failure, No PCR products.
  • A: Numbers 13 and 14: Success, PCR products have the expected size.
  • B: All 6 extractions were successful.
  • C: Number 1: successful concentration of pOsV230's PCR product.
  • C: Number 2 had no migration.

Protocol

Photo of the Day

Paris Saclay 21 july.jpg

People there:

  • Instructors and advisors: Solenne and Sylvie.
  • Students: Arnaud, Fabio, Juliette, Mathieu, Marie, Pierre, Romain, Sean and Terry.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/21"