Overview

Anti-sense RNA is studied actively over the world. However, reliable method for gene silencing has not been clear. It is hard to find efficient sequence of anti-sense RNA and to synthesize new anti-sense fragment in keeping with your target gene.

fig1. you have to change anti-sense RNA conforming with each target gene.

We had a good idea! It is useful to use common anti-sense for different target gene.

fig2. Image of common anti-sense RNA effects.

Here we found that anti-sense-RBS(B0034) fragment, that is used by igemer commonly, work to silence several proteins. We synthesized anti-sense B0034 fragment, igemer often use that RBS. Regardless of target gene, only one anti-sense fragment works various proteins on common RBS, B0034.

We also synthesized anti-sense-B0032 fragment in order to achieve specific silencing gene. The combination of anti-sense fragment and RBS, that locates upstream of target gene makes change silencing protein.

Specific anti-sense-RBS fragment help you save labor to make new anti-sense RNA for each target genes. Fortunately, igem HokkaidoU team select tractable RBS for designing anti-sense-RBS fragments. You can use our anti-sense fragments without resynthesize constructs you had. You only have to do is add our anti-sense fragment to construct had target gene!!

fig3. Anti-sense B0034 has specific effects to B0034, RBS.