Team:SCUT-China/Safety

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:SCUT-China/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:SCUT-China"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:SCUT-China/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=SCUT-China"style="color:#000000"> Official Team Profile </a></td>
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<p class="head">Safety</p>
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<a href="https://2014.igem.org/Team:SCUT-China/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:SCUT-China/Parts"style="color:#000000"> Parts</a></td>
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<p><span class="title">The chassis organisms we used for this project</span></p>
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<a href="https://2014.igem.org/Team:SCUT-China/Modeling"style="color:#000000"> Modeling</a></td>
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<td class="name">Species name (including strain)</td>
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<td class="name">Risk Group</td>
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<td class="name">Disease risk to humans?</td>
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<a href="https://2014.igem.org/Team:SCUT-China/Notebook"style="color:#000000"> Notebook</a></td>
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<td>E. coli(K12) BL21</td>
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<td>1</td>
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<td>Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E.coli is not considered a human or animal pathogen nor is it toxicogenic.</td>
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<a href="https://2014.igem.org/Team:SCUT-China/Safety"style=" color:#000000"> Safety </a></td>
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<td>E. coli(K12) TOP10</td>
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<td>1</td>
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<td>Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E.coli is not considered a human or animal pathogen nor is it toxicogenic.</td>
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<a href="https://2014.igem.org/Team:SCUT-China/Attributions"style="color:#000000"> Attributions </a></td>
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<td>E. coli(K12) DH5alpha</td>
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<td>1</td>
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<td>Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E.coli is not considered a human or animal pathogen nor is it toxicogenic.</td>
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<td>Saccharopolyspora erythraea</td>
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<td>1</td>
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<td>No</td>
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<td>Bacillus subtilis 168</td>
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<td>1</td>
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<td>No</td>
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<p><span class="title">List and description of all new or modified coding regions we used</span></p>
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<td class="name">Part number/name</td>
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<td class="name">What species does this part originally come from?</td>
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<td class="name">Risk Group</td>
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<td class="name">Natural function of part</td>
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<td class="name">How will you use it?</td>
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<td>BBa_K1371000 (PKS)</td>
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<td>Saccharopolyspora erythraea</td>
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<td>1</td>
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<td>Produce the macrolide antibiotic erythromycin</td>
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<td>This part produces the macrolide antibiotic which our promoter is designed to detect</td>
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<td>BBa_K1371011 (sfp)</td>
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<td>Bacillus subtilis 168</td>
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<td>1</td>
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<td>Code for PPTase</td>
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<td>This part is code for PPTase which can activate ATP</td>
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<td>BBa_K1371012 (birA)</td>
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<td>E. coli(K12)  BL21</td>
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<td>1</td>
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<td>Code for biotin ligase</td>
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<td>Enhance the activity of pccB upon coexpression</td>
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<tr><td > <h3> Welcome! </h3></td>
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<td>BBa_K1371013 (pccB)</td>
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<td>Streptomyces coelicolor</td>
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<td>1</td>
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<td>carboxyl transferase, β subunit of pcc</td>
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<td>This part produces the propionyl-CoA with combination of accA</td>
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<td>BBa_K1371014 (accA)</td>
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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<td>Streptomyces coelicolor</td>
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<td>1</td>
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<td>acetyl-CoA carboxylase, biotinylated  α subunit of pcc</td>
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<td>This part produces the propionyl-CoA with combination of pccB</td>
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<td>BBa_K1371016 (docking domain1)</td>
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<td>Saccharopolyspora erythraea</td>
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<td>1</td>
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<td>Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides</td>
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<td>efficient transfer of polyketide intermediates between polypeptides</td>
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<td>BBa_K1371017 (docking domain2)</td>
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<td>Saccharopolyspora erythraea</td>
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<td>1</td>
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<td>Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides</td>
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<td>efficient transfer of polyketide intermediates between polypeptides</td>
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<br>
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<h3> Edit this page!</h3>
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<td>BBa_K1371018 (docking domain3)</td>
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<td>Saccharopolyspora erythraea</td>
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Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
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<td>1</td>
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<td>Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides</td>
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<li> <a href=" ">Learn about lab Safety for Today</a></li>  
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<td>efficient transfer of polyketide intermediates between polypeptides</td>
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<li> <a href="">Learn about Safety for the future of your project.</a></li>  
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<h3> Your Lab </h3>
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<td>BBa_K1371019 (docking domain4)</td>
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<td>Saccharopolyspora erythraea</td>
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>  
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<td>Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides</td>
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<td>efficient transfer of polyketide intermediates between polypeptides</td>
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Image:Example2_Lab_1.png|The building our lab is in!
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Image:Example2_Lab_2.png|The inside of our lab!
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Image:Example2_Lab_3.png|Team Member 3 doing an experiment
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Image:Example2_Lab_4.png|Working in biosafety cabinets
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Image:Example2_Lab_5.png|Team all gloved up and ready for work!
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Image:Example2_Lab_6.png|Equipment that we use to do SCIENCE!
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Image:Example2_Lab_7.png|We decorated this part of our lab
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Image:Example2_Lab_8.png|Whatever else you want
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<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
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<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
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<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
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<td>BBa_K1371020 (docking domain5)</td>
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<td>Saccharopolyspora erythraea</td>
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<td>1</td>
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<td>Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides</td>
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<td>efficient transfer of polyketide intermediates between polypeptides</td>
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<td>BBa_K1371021 (docking domain6)</td>
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<td>Saccharopolyspora erythraea</td>
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<td>1</td>
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<td>Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides</td>
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<td>efficient transfer of polyketide intermediates between polypeptides</td>
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<br/>
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<p>Do the biological materials used in our lab work pose any of the following risks?
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Every species we worked with belongs to BL1. They do no harm to human. Experimenters is careful not to release any parts to the public. And even the worst situation happens as it is released, it will hardly bring risks.
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</p>
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<p><span class="title">a)</span>
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<span class="bold">Risks to the safety and health of team members, or other people working in the lab:</span><br/>
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Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E.coli is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E.coli in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections.<br/><br/>
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<span class="title">b)</span>
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<span class="bold">Risks to the environment (from waste disposal, or from materials escaping from your lab):</span><Br/>
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The plasmids we designed cannot function outside certain cell lines in the laboratory, And the cell we used is very fragile and it will soon die leaving the culture medium. So it will not bring contamination to the environment.<br/><br/>
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<span class="title">c)</span>
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<span class="bold">Risks to security through malicious mis-use by individuals, groups, or countries:</span><Br/>
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All the biological materials used in our lab are safe, and all the products of the coding sequences are innocuous to human, so the risk of malicious misuse is low.<Br/><br/>
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A regular safety briefing and a lecture about the basics concerning of biotechnology and genetic engineering are basic elements of our education at the SCUT.
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The handling of biological material and safety aspects of chemicals are explained.
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A safety briefing was held for all iGEM students by Zhenwu Zhang who is the responsible person for our lab safety.
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Latest revision as of 09:02, 14 October 2014


Safety

The chassis organisms we used for this project


Species name (including strain) Risk Group Disease risk to humans?
E. coli(K12) BL21 1 Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E.coli is not considered a human or animal pathogen nor is it toxicogenic.
E. coli(K12) TOP10 1 Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E.coli is not considered a human or animal pathogen nor is it toxicogenic.
E. coli(K12) DH5alpha 1 Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E.coli is not considered a human or animal pathogen nor is it toxicogenic.
Saccharopolyspora erythraea 1 No
Bacillus subtilis 168 1 No

List and description of all new or modified coding regions we used


Part number/name What species does this part originally come from? Risk Group Natural function of part How will you use it?
BBa_K1371000 (PKS) Saccharopolyspora erythraea 1 Produce the macrolide antibiotic erythromycin This part produces the macrolide antibiotic which our promoter is designed to detect
BBa_K1371011 (sfp) Bacillus subtilis 168 1 Code for PPTase This part is code for PPTase which can activate ATP
BBa_K1371012 (birA) E. coli(K12) BL21 1 Code for biotin ligase Enhance the activity of pccB upon coexpression
BBa_K1371013 (pccB) Streptomyces coelicolor 1 carboxyl transferase, β subunit of pcc This part produces the propionyl-CoA with combination of accA
BBa_K1371014 (accA) Streptomyces coelicolor 1 acetyl-CoA carboxylase, biotinylated α subunit of pcc This part produces the propionyl-CoA with combination of pccB
BBa_K1371016 (docking domain1) Saccharopolyspora erythraea 1 Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides efficient transfer of polyketide intermediates between polypeptides
BBa_K1371017 (docking domain2) Saccharopolyspora erythraea 1 Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides efficient transfer of polyketide intermediates between polypeptides
BBa_K1371018 (docking domain3) Saccharopolyspora erythraea 1 Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides efficient transfer of polyketide intermediates between polypeptides
BBa_K1371019 (docking domain4) Saccharopolyspora erythraea 1 Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides efficient transfer of polyketide intermediates between polypeptides
BBa_K1371020 (docking domain5) Saccharopolyspora erythraea 1 Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides efficient transfer of polyketide intermediates between polypeptides
BBa_K1371021 (docking domain6) Saccharopolyspora erythraea 1 Code for docking domain which is required efficient transfer of polyketide intermediates between polypeptides efficient transfer of polyketide intermediates between polypeptides

Do the biological materials used in our lab work pose any of the following risks? Every species we worked with belongs to BL1. They do no harm to human. Experimenters is careful not to release any parts to the public. And even the worst situation happens as it is released, it will hardly bring risks.


a) Risks to the safety and health of team members, or other people working in the lab:
Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E.coli is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E.coli in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections.

b) Risks to the environment (from waste disposal, or from materials escaping from your lab):
The plasmids we designed cannot function outside certain cell lines in the laboratory, And the cell we used is very fragile and it will soon die leaving the culture medium. So it will not bring contamination to the environment.

c) Risks to security through malicious mis-use by individuals, groups, or countries:
All the biological materials used in our lab are safe, and all the products of the coding sequences are innocuous to human, so the risk of malicious misuse is low.

A regular safety briefing and a lecture about the basics concerning of biotechnology and genetic engineering are basic elements of our education at the SCUT. The handling of biological material and safety aspects of chemicals are explained. A safety briefing was held for all iGEM students by Zhenwu Zhang who is the responsible person for our lab safety.

Retrieved from "http://2014.igem.org/Team:SCUT-China/Safety"