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Template:Team:USyd-Australia/Calendar/Events List
From 2014.igem.org
(Difference between revisions)
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{ title: "Construction of pUS204, Transformation: pUS204 into top10 cells", | { title: "Construction of pUS204, Transformation: pUS204 into top10 cells", | ||
start: new Date(2014,30,7), | start: new Date(2014,30,7), | ||
| - | description: "Details: Transformation (competent top10, pUS204). Incubated on AmpR plates for 2hrs<br><br>Result: Some AmpR colonies present, no blue colonies detected<br><br>Conclusion: pUS024 did not successfully transform, | + | description: "Details: Transformation (competent top10, pUS204). Incubated on AmpR plates for 2hrs<br><br>Result: Some AmpR colonies present, no blue colonies detected<br><br>Conclusion: pUS024 did not successfully transform, "far unlikely positive control worked" <br><br>- ?"}, |
{ title: "construction of pUS201, see notebook: second GA and transformation successful???", | { title: "construction of pUS201, see notebook: second GA and transformation successful???", | ||
start: new Date(2014,0,8), | start: new Date(2014,0,8), | ||
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start: new Date(2014,4,8), | start: new Date(2014,4,8), | ||
description: "Details: Incubated overnight to finish later<br><br>Result: Yield 5.7ng/ul, 1.61 260/280<br><br>Conclusion: Yield too low to use<br><br>- Jeanne & Callum"}, | description: "Details: Incubated overnight to finish later<br><br>Result: Yield 5.7ng/ul, 1.61 260/280<br><br>Conclusion: Yield too low to use<br><br>- Jeanne & Callum"}, | ||
| - | { title: "Checking IntI1 from SamR | + | { title: "Checking IntI1 from SamR's construct, Restriction digest: SamR's pSB1C3+ARApBAD", |
start: new Date(2014,7,8), | start: new Date(2014,7,8), | ||
description: "Details: Restriction digest (EcoR1+Pst1), incubated at 37 C for 1hr<br><br>Result: Yield: 4.46 ug/uL. Stored in -20 C fridge<br><br>Conclusion: Yield much higher than estimate provided by SamR (50ng/uL). Useable<br><br>- Rokiah"}, | description: "Details: Restriction digest (EcoR1+Pst1), incubated at 37 C for 1hr<br><br>Result: Yield: 4.46 ug/uL. Stored in -20 C fridge<br><br>Conclusion: Yield much higher than estimate provided by SamR (50ng/uL). Useable<br><br>- Rokiah"}, | ||
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start: new Date(2014,11,8), | start: new Date(2014,11,8), | ||
description: "Details: Restriction Digest (uncut, EcoR1, HindIII, HindIII+BamHI)<br><br>Result: gel????? Results???<br><br>- ?"}, | description: "Details: Restriction Digest (uncut, EcoR1, HindIII, HindIII+BamHI)<br><br>Result: gel????? Results???<br><br>- ?"}, | ||
| - | { title: "Checking IntI1 from SamR | + | { title: "Checking IntI1 from SamR's construct, Checking: SamR's digests on gel electrohoresis", |
start: new Date(2014,11,8), | start: new Date(2014,11,8), | ||
description: "Details: Gel electrophoresis (Spe1, uncut) at 120V for 1hr. Stained in gel red for ~30min.<br><br>Result: [12.08 SamR gel]. Cut lanes travelled further than uncut, as expected.<br><br>Conclusion: NOTE:: notes at the end cannot be read<br><br>- Rokiah"}, | description: "Details: Gel electrophoresis (Spe1, uncut) at 120V for 1hr. Stained in gel red for ~30min.<br><br>Result: [12.08 SamR gel]. Cut lanes travelled further than uncut, as expected.<br><br>Conclusion: NOTE:: notes at the end cannot be read<br><br>- Rokiah"}, | ||
| - | { title: "Making IntI1 for pUS203, Restriction Digest: extracting SamR | + | { title: "Making IntI1 for pUS203, Restriction Digest: extracting SamR's AraC-pBAD from pSB1C3", |
start: new Date(2014,12,8), | start: new Date(2014,12,8), | ||
description: "Details: pSB1C3 with AraC-pBAD was incubated with *specific enzyme* at 80 C for 20 min. The enzyme was heat-killed<br><br>- andy"}, | description: "Details: pSB1C3 with AraC-pBAD was incubated with *specific enzyme* at 80 C for 20 min. The enzyme was heat-killed<br><br>- andy"}, | ||
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{ title: "Checkin pUS203, Patch plate Int1I-2 and SamR construct", | { title: "Checkin pUS203, Patch plate Int1I-2 and SamR construct", | ||
start: new Date(2014,10,9), | start: new Date(2014,10,9), | ||
| - | description: "Details: GA product cultures transferred to patch plates using 25x | + | description: "Details: GA product cultures transferred to patch plates using 25x "small" colonies and 25x"large" colonies.<br><br>Result: Colonies to be screened with junction PCR. Why patch plate?<br><br>Conclusion: ?<br><br>- ?"}, |
| - | { title: "Checking Pus203, Preperation of colony PCR of PUS203: of the | + | { title: "Checking Pus203, Preperation of colony PCR of PUS203: of the "small" and "large" colonies on patch plate", |
start: new Date(2014,11,9), | start: new Date(2014,11,9), | ||
description: "Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>"}, | description: "Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>"}, | ||
| - | { title: "Checking Pus203, colony PCR of PUS203: of the | + | { title: "Checking Pus203, colony PCR of PUS203: of the "small" and "large" colonies on patch plate", |
start: new Date(2014,14,9), | start: new Date(2014,14,9), | ||
description: "Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>Result: Expected fragment size was 473bp. No data received. ????<br><br>Conclusion: ?? Re-do?<br><br>- rokiah"}, | description: "Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>Result: Expected fragment size was 473bp. No data received. ????<br><br>Conclusion: ?? Re-do?<br><br>- rokiah"}, | ||
Revision as of 07:02, 14 October 2014
