Team:USTC-China/project

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                <a href="motion">Motion Control </a>
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                <a href="conjugation">Conjugation </a>
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                <a href="killswitch">Kill switch </a>
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                <a href="rnalogic">RNA logic control </a>
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    <div class="title"><h1>Overview</h1></div>
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<div class="title"><h1>Parts</h1></div>
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      <p>Modern "lithography" has made images vividly printed on pages and animations vigorously projected on screens. <br />
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<h2 id="partsthatwedesign">Parts that we design</h2>
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        From that point, we move a step forward. Our project aims at conceiving and constructing a colorful bio-imaging system, on which once you project an image, ideally, the bio-system would exactly print it out on the substrate. <br />
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        To accomplish this, novel bio-bricks(light sensors &amp; color printers), Caulobacter crescentus(a sticky bacteria)and riboswitches were utilized to conceptually render it colorful,clear and compact. The results did prove this idea sound in principle, although further evaluation is required. <br />
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<h4>This year we submit 20 designed parts, 18 of them were provided to the Parts Registry. These parts can be divided into the following kinds:</h4>
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        We have managed to build light sensing circuits of three color and a motion control device in C.crescentus. furthermore, RNA logical gates were built in vivo as substitutes for certain parts with hopefully unprecedented effectiveness.</p>
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<h4>Blue, red and light sensing-imaging system</h4>
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<li><a href="http://parts.igem.org/Part:BBa_K1363400"> BBa_K1363400 </a>: combination of R0010-K592016-B0015-K502020 </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363401"> BBa_K1363401 </a>: the downstream passway of the red color system</li>
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<h4>2.Motion control of <em>Caulobacter crescentus</em></h4>
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<li><a href="http://parts.igem.org/Part:BBa_K1363000"> BBa_K1363000 </a>: DgrA, the receptor of c-di-GMP </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363001"> BBa_K1363001 </a>: DgrB, the receptor of c-di-GMP</li>
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<h4>3.Biosynthesis of  holdfast of <em>Caulobacter crescentus</em></h4>
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<li><a href="http://parts.igem.org/Part:BBa_K1363002"> BBa_K1363002 </a>: HfiA the Inhibitor of synthesis of polysaccharose </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363005"> BBa_K1363005 </a>: R0082-HfiA the HfiA protein express passway which can be regulated by ligh</li>
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<h4>4.RNA logic control system</h4>
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<li><a href="http://parts.igem.org/Part:BBa_K1363610"> BBa_K1363610 </a>: key-1-of-or of RNA logic gates </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363611"> BBa_K1363611 </a>: key-1 of gate-of-or of RNA logic gates </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363606"> BBa_K1363606 </a>: And Key 1 </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363607"> BBa_K1363607 </a>: And Key 2 </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363609"> BBa_K1363609 </a>: Or Key 1 </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363610"> BBa_K1363610 </a>: Or Key 2 </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363602"> BBa_K1363602 </a>: key of yes of RNA logic gates </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363603"> BBa_K1363603 </a>: gate of no of RNA logic gates </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363604"> BBa_K1363604 </a>: key of no of RNA logic gates </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363605"> BBa_K1363605 </a>: gate of and of RNA logic gates </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363601"> BBa_K1363601 </a>: the gate of yes of RNA logic gates </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363600"> BBa_K1363600 </a>: a aptamyze regulator sensetive to Theophylline </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363500"> BBa_K1363500 </a>: a aptamyze regulator sensetive to Theophylline</li>
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<h4>5.Kill switch based on LALF regulation(Didn't submit the real part)</h4>
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<li><a href="http://parts.igem.org/Part:BBa_K1363200"> BBa_K1363200 </a>: Anti-LPS factor(LALF) regulated by lacI </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363201"> BBa_K1363201 </a>: Anti-LPS factor(LALF) regulated by IPTG</li>
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<h4>6.Conjugation parts from S17-1 to other bacteria.</h4>
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<li><a href="http://parts.igem.org/Part:BBa_K1363003"> BBa_K1363003 </a>: OriT-K592016 the blue sensor which can be transfered into other bacterias by S17-1 </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363004"> BBa_K1363004 </a>: the downstream passway of the blue light system which can be transfered into Caulobacter crescentus </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1363006"> BBa_K1363006 </a>: an experimental part used to test the conjugation</li>
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Revision as of 05:48, 14 October 2014

Parts

Parts that we design

This year we submit 20 designed parts, 18 of them were provided to the Parts Registry. These parts can be divided into the following kinds:

Blue, red and light sensing-imaging system

2.Motion control of Caulobacter crescentus

3.Biosynthesis of holdfast of Caulobacter crescentus

  • BBa_K1363002 : HfiA the Inhibitor of synthesis of polysaccharose
  • BBa_K1363005 : R0082-HfiA the HfiA protein express passway which can be regulated by ligh

4.RNA logic control system

5.Kill switch based on LALF regulation(Didn't submit the real part)

6.Conjugation parts from S17-1 to other bacteria.

  • BBa_K1363003 : OriT-K592016 the blue sensor which can be transfered into other bacterias by S17-1
  • BBa_K1363004 : the downstream passway of the blue light system which can be transfered into Caulobacter crescentus
  • BBa_K1363006 : an experimental part used to test the conjugation

Lots of thanks to our sponsors, who help us to achieve what we have today

© 2014 USTC-iGEM Design. Distributed under BY-SA 3.0

Retrieved from "http://2014.igem.org/Team:USTC-China/project"