Template:Team:USyd-Australia/Calendar/Events List

From 2014.igem.org

(Difference between revisions)
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{ title: 'Plasmid prep of psB6A1, Checking: finish digests, gel electrophoresis of digests',
{ title: 'Plasmid prep of psB6A1, Checking: finish digests, gel electrophoresis of digests',
start: new Date(2014,9,7),
start: new Date(2014,9,7),
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description: 'Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. pSB6A1 yielded 5104.1ng/uL DNA (A260/280) 1.62.<br><br>Conclusion: Possible protein contamination to samples<br><br>-  Abi'},
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description: 'Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. pSB6A1 yielded 5104.1ng/uL DNA &amp; (A260/280) 1.62.<br><br>Conclusion: Possible protein contamination to samples<br><br>-  Abi'},
{ title: 'Plasmid prep of pSB6A1, Re-doing digest',
{ title: 'Plasmid prep of pSB6A1, Re-doing digest',
start: new Date(2014,10,7),
start: new Date(2014,10,7),
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{ title: 'Plasmid prep of pUS44, Re-doing digest',
{ title: 'Plasmid prep of pUS44, Re-doing digest',
start: new Date(2014,16,7),
start: new Date(2014,16,7),
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description: 'Details: Restriction digest. Did the protocol change?<br><br>Conclusion: Nanospec: 395.5ng/mL DNA yield<br><br>-  Abi Tom'},
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description: 'Details: Restriction digest. Did the protocol change?<br><br>Conclusion: Nanospec: 395.5ng/mL DNA yield<br><br>-  Abi &amp; Tom'},
{ title: 'Construction of pUS201, PCR of pSB6A1 using iGEM401/402 primers ',
{ title: 'Construction of pUS201, PCR of pSB6A1 using iGEM401/402 primers ',
start: new Date(2014,24,7),
start: new Date(2014,24,7),
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{ title: 'construction of pUS201, re-doing pSB1C3 PCR with iGEM1403/1404',
{ title: 'construction of pUS201, re-doing pSB1C3 PCR with iGEM1403/1404',
start: new Date(2014,3,8),
start: new Date(2014,3,8),
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description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in the fridge<br><br>-  Jeanne Callum'},
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description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in the fridge<br><br>-  Jeanne &amp; Callum'},
{ title: 'construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ',
{ title: 'construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ',
start: new Date(2014,4,8),
start: new Date(2014,4,8),
-
description: 'Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>-  Jeanne Callum'},
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description: 'Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>-  Jeanne &amp; Callum'},
-
{ title: 'Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies',
+
-
start: new Date(2014,4,8),
+
-
description: 'Details: Incubated overnight to finish later<br><br>Result: Yield 5.7ng/ul, 1.61 260/280<br><br>Conclusion: Yield too low to use<br><br>-  Jeanne  Callum'},
+
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{ title: 'Checking IntI1 from SamR's construct, Restriction digest: SamR's pSB1C3+ARApBAD',
+
-
start: new Date(2014,7,8),
+
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description: 'Details: Restriction digest (EcoR1+Pst1), incubated at 37 C for 1hr<br><br>Result: Yield: 4.46 ug/uL. Stored in -20 C fridge<br><br>Conclusion: Yield much higher than estimate provided by SamR (50ng/uL). Useable<br><br>-  Rokiah'},
+
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{ title: 'Checking pUS201 construct, Checking pUS201 with digests',
+
-
start: new Date(2014,11,8),
+
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description: 'Details: Restriction Digest (uncut, EcoR1, HindIII, HindIII+BamHI)<br><br>Result: gel????? Results???<br><br>-  ?'},
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{ title: 'Checking IntI1 from SamR's construct, Checking: SamR's digests on gel electrohoresis',
{ title: 'Checking IntI1 from SamR's construct, Checking: SamR's digests on gel electrohoresis',
start: new Date(2014,11,8),
start: new Date(2014,11,8),
Line 98: Line 89:
{ title: 'construction of PUS204, Junction PCR to check whether pUS204 has been ligated successfully',
{ title: 'construction of PUS204, Junction PCR to check whether pUS204 has been ligated successfully',
start: new Date(2014,8,9),
start: new Date(2014,8,9),
-
description: 'Details: Junction PCR with iGEM1407/iGEM1408 primers and Taq<br><br>Result: Products plated for... why?<br><br>-  Tom'},
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description: 'Details: Junction PCR with iGEM1407/iGEM1408 primers and Taq<br><br>Result: Products plated for…why?<br><br>-  Tom'},
{ title: 'Construction of pUS203, Checking: heat-killing and running a gel of products',
{ title: 'Construction of pUS203, Checking: heat-killing and running a gel of products',
start: new Date(2014,9,9),
start: new Date(2014,9,9),

Revision as of 04:51, 14 October 2014