|
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| Line 66: |
Line 66: |
| | start: new Date(2014,4,8), | | start: new Date(2014,4,8), |
| | description: 'Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>- Jeanne & Callum'}, | | description: 'Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>- Jeanne & Callum'}, |
| - | { title: 'Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies',
| + | |
| - | start: new Date(2014,4,8),
| + | |
| - | description: 'Details: Incubated overnight to finish later<br><br>Result: Yield 5.7ng/ul, 1.61 260/280<br><br>Conclusion: Yield too low to use<br><br>- Jeanne & Callum'},
| + | |
| - | { title: 'Checking IntI1 from SamR's construct, Restriction digest: SamR's pSB1C3+ARApBAD',
| + | |
| - | start: new Date(2014,7,8),
| + | |
| - | description: 'Details: Restriction digest (EcoR1+Pst1), incubated at 37 C for 1hr<br><br>Result: Yield: 4.46 ug/uL. Stored in -20 C fridge<br><br>Conclusion: Yield much higher than estimate provided by SamR (50ng/uL). Useable<br><br>- Rokiah'},
| + | |
| - | { title: 'Checking pUS201 construct, Checking pUS201 with digests',
| + | |
| - | start: new Date(2014,11,8),
| + | |
| - | description: 'Details: Restriction Digest (uncut, EcoR1, HindIII, HindIII+BamHI)<br><br>Result: gel????? Results???<br><br>- ?'},
| + | |
| | ] | | ] |
| | }); | | }); |
"
