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| | { title: 'Checking pUS201 construct, Re-checking the pUS201 by restriction digestion', | | { title: 'Checking pUS201 construct, Re-checking the pUS201 by restriction digestion', |
| | start: new Date(2014,3,9), | | start: new Date(2014,3,9), |
| - | description: 'Details: Restriction digestion (EcoR1, NdeI, NdeI/BamHI, NdeI/HindIII)<br><br>'},
| |
| - | { title: 'construction of PUS204, Junction PCR to check whether pUS204 has been ligated successfully',
| |
| - | start: new Date(2014,8,9),
| |
| - | description: 'Details: Junction PCR with iGEM1407/iGEM1408 primers and Taq<br><br>Result: Products plated for... why?<br><br>- Tom'},
| |
| - | { title: 'Construction of pUS203, Checking: heat-killing and running a gel of products',
| |
| - | start: new Date(2014,9,9),
| |
| - | description: 'Details: Heat killed construct at 80 C for 20 min. Ran a gel electrophoresis of products wfor 100V for 1hr.<br><br>Result: Gel failed.<br><br>Conclusion: Gibson Assembly attempted anyway, gel fail was not indication of no construct<br><br>- Tom'},
| |
| - | { title: 'Construction of pUS201, Plating out Pus201 Gibson Assembly',
| |
| - | start: new Date(2014,9,9),
| |
| - | description: 'Details: Plated out GA products onto Amp plates.<br><br>- Tom'},
| |
| - | { title: 'Construction of pUS204, Junction PCR gel',
| |
| - | start: new Date(2014,9,9),
| |
| - | description: 'Details: Gel electrophoresis with PCR products (overnight pSB1C3/aeBlue cultures, 1hr growth cultures, no plasmid colony, no template control)<br><br>Result: ??<br><br>Conclusion: ??<br><br>'},
| |
| - | { title: 'Checkin pUS203, Patch plate Int1I-2 and SamR construct',
| |
| - | start: new Date(2014,10,9),
| |
| - | description: 'Details: GA product cultures transferred to patch plates using 25x "small" colonies and 25x"large" colonies.<br><br>Result: Colonies to be screened with junction PCR. Why patch plate?<br><br>Conclusion: ?<br><br>- ?'},
| |
| - | { title: 'Checking Pus203, Preperation of colony PCR of PUS203: of the "small" and "large" colonies on patch plate',
| |
| - | start: new Date(2014,11,9),
| |
| - | description: 'Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>'},
| |
| - | { title: 'Checking Pus203, colony PCR of PUS203: of the "small" and "large" colonies on patch plate',
| |
| - | start: new Date(2014,14,9),
| |
| - | description: 'Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>Result: Expected fragment size was 473bp. No data received. ????<br><br>Conclusion: ?? Re-do?<br><br>- rokiah'},
| |
| | ] | | ] |
| | }); | | }); |
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