Template:Team:USyd-Australia/Calendar/Events List

From 2014.igem.org

(Difference between revisions)
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{ title: 'Plasmid Prep psB6A1, Finish',
{ title: 'Plasmid Prep psB6A1, Finish',
start: new Date(2014,8,7),
start: new Date(2014,8,7),
-
description: 'Details: Finish<br><br>Result: Finish<br><br>Conclusion: Finish<br><br>-  Rokiah
+
description: 'Result: Plasmid prep yielded 4x100uL tubes<br><br>-  Rokiah'},
{ title: 'Plasmid prep of pUS44, Checking: Setup restriction digest and incubate',
{ title: 'Plasmid prep of pUS44, Checking: Setup restriction digest and incubate',
start: new Date(2014,8,7),
start: new Date(2014,8,7),
-
description: 'Details: Checking: Setup restriction digest and incubate<br><br>Result: Checking: Setup restriction digest and incubate<br><br>Conclusion: Checking: Setup restriction digest and incubate<br><br>-  Abi
+
description: 'Details: restriction digest to check plasmids (EcoR1, Ssp1, null). Incubated at 37C overnight.<br><br>-  Abi'},
{ title: 'Plasmid prep of pUS44, Checking: finish digests, gel electrophoresis of digests',
{ title: 'Plasmid prep of pUS44, Checking: finish digests, gel electrophoresis of digests',
start: new Date(2014,9,7),
start: new Date(2014,9,7),
-
description: 'Details: Checking: finish digests, gel electrophoresis of digests<br><br>Result: Checking: finish digests, gel electrophoresis of digests<br><br>Conclusion: Checking: finish digests, gel electrophoresis of digests<br><br>-  Abi
+
description: 'Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. <br><br>-  Abi'},
{ title: 'Plasmid prep of psB6A1, Checking: finish digests, gel electrophoresis of digests',
{ title: 'Plasmid prep of psB6A1, Checking: finish digests, gel electrophoresis of digests',
start: new Date(2014,9,7),
start: new Date(2014,9,7),
-
description: 'Details: Checking: finish digests, gel electrophoresis of digests<br><br>Result: Checking: finish digests, gel electrophoresis of digests<br><br>Conclusion: Checking: finish digests, gel electrophoresis of digests<br><br>-  Abi
+
description: 'Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. pSB6A1 yielded 5104.1ng/uL DNA & (A260/280) 1.62.<br><br>Conclusion: Possible protein contamination to samples<br><br>-  Abi'},
{ title: 'Plasmid prep of pSB6A1, Re-doing digest',
{ title: 'Plasmid prep of pSB6A1, Re-doing digest',
start: new Date(2014,10,7),
start: new Date(2014,10,7),
-
description: 'Details: Re-doing digest<br><br>Result: Re-doing digest<br><br>Conclusion: Re-doing digest<br><br>-  Jeanne
+
description: 'Details: Restriction digest to check plasmids (EcoR1, EcoR1+Pst1, EcoR1+Nde1). <br><br>-  Jeanne'},
{ title: 'Plasmid prep of pUS44, Re-doing digest',
{ title: 'Plasmid prep of pUS44, Re-doing digest',
start: new Date(2014,14,7),
start: new Date(2014,14,7),
-
description: 'Details: Re-doing digest<br><br>Result: Re-doing digest<br><br>Conclusion: Re-doing digest<br><br>-  Abi
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description: 'Details: Restriction digest to check plasmids (EcoR1, EcoR1+Pst1, EcoR1+Nde1). <br><br>-  Abi'},
{ title: 'Plasmid prep of pUS44, Re-doing digest',
{ title: 'Plasmid prep of pUS44, Re-doing digest',
start: new Date(2014,16,7),
start: new Date(2014,16,7),
-
description: 'Details: Re-doing digest<br><br>Result: Re-doing digest<br><br>Conclusion: Re-doing digest<br><br>-  Abi & Tom
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description: 'Details: Restriction digest. Did the protocol change?<br><br>Conclusion: Nanospec: 395.5ng/mL DNA yield<br><br>-  Abi & Tom'},
{ title: 'Construction of pUS201, PCR of pSB6A1 using iGEM401/402 primers ',
{ title: 'Construction of pUS201, PCR of pSB6A1 using iGEM401/402 primers ',
start: new Date(2014,24,7),
start: new Date(2014,24,7),
-
description: 'Details: PCR of pSB6A1 using iGEM401/402 primers <br><br>Result: PCR of pSB6A1 using iGEM401/402 primers <br><br>Conclusion: PCR of pSB6A1 using iGEM401/402 primers <br><br>-  Callum
+
description: 'Details: PCR Master mix with 1401/1402 primers. Used iGEM pSB6A1. Ran on regular program PCR ~2HRS<br><br>Result: Yield 1018.6ng/uL and low 260/280<br><br>Conclusion: ?<br><br>-  Callum'},
{ title: 'Construction of pUS201, Checking: finish pcr, gel electrophoresis of digests',
{ title: 'Construction of pUS201, Checking: finish pcr, gel electrophoresis of digests',
start: new Date(2014,24,7),
start: new Date(2014,24,7),
-
description: 'Details: Checking: finish pcr, gel electrophoresis of digests<br><br>Result: Checking: finish pcr, gel electrophoresis of digests<br><br>Conclusion: Checking: finish pcr, gel electrophoresis of digests<br><br>-  Callum
+
description: 'Details: Gel electrophoresis (100V, 400mA, 1hr). Stained in gel red for 30min<br><br>Result: Expected fragment size of 2.6kB from pSB6A1<br><br>Conclusion: Primers amplified expected product from pSB6A1<br><br>-  Callum'},
{ title: 'Construction of pUS204, Restriction digest of pSB1C3',
{ title: 'Construction of pUS204, Restriction digest of pSB1C3',
start: new Date(2014,29,7),
start: new Date(2014,29,7),
-
description: 'Details: Restriction digest of pSB1C3<br><br>Result: Restriction digest of pSB1C3<br><br>Conclusion: Restriction digest of pSB1C3<br><br>-  Callum
+
description: 'Details: Restriction digest (EcoR1+Pst1)<br><br>Result: Yield 25ng/mL<br><br>Conclusion: Enough to use for gibson assembly<br><br>-  Callum'},
{ title: 'Construction of pUS201, PCR of pSB1C3 using iGEM403/1404 primers ',
{ title: 'Construction of pUS201, PCR of pSB1C3 using iGEM403/1404 primers ',
start: new Date(2014,30,7),
start: new Date(2014,30,7),
-
description: 'Details: PCR of pSB1C3 using iGEM403/1404 primers <br><br>Result: PCR of pSB1C3 using iGEM403/1404 primers <br><br>Conclusion: PCR of pSB1C3 using iGEM403/1404 primers <br><br>-  Callum
+
description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Yield 329.4ng/uL, low 260/280<br><br>-  Callum'},
{ title: 'Construction of pUS204, Transformation: pUS204 into top10 cells',
{ title: 'Construction of pUS204, Transformation: pUS204 into top10 cells',
start: new Date(2014,30,7),
start: new Date(2014,30,7),
-
description: 'Details: Transformation: pUS204 into top10 cells<br><br>Result: Transformation: pUS204 into top10 cells<br><br>Conclusion: Transformation: pUS204 into top10 cells<br><br>-  ?
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description: 'Details: Transformation (competent top10, pUS204). Incubated on AmpR plates for 2hrs<br><br>Result: Some AmpR colonies present, no blue colonies detected<br><br>Conclusion: pUS024 did not successfully transform, "far unlikely positive control worked" <br><br>-  ?'},
{ title: 'construction of pUS201, see notebook: second GA and transformation successful???',
{ title: 'construction of pUS201, see notebook: second GA and transformation successful???',
start: new Date(2014,0,8),
start: new Date(2014,0,8),
-
description: 'Details: see notebook: second GA and transformation successful???<br><br>Result: see notebook: second GA and transformation successful???<br><br>Conclusion: see notebook: second GA and transformation successful???<br><br>- 
+
description: ''},
{ title: 'construction of pUS201, re-doing pSB1C3 PCR with iGEM1403/1404',
{ title: 'construction of pUS201, re-doing pSB1C3 PCR with iGEM1403/1404',
start: new Date(2014,3,8),
start: new Date(2014,3,8),
-
description: 'Details: re-doing pSB1C3 PCR with iGEM1403/1404<br><br>Result: re-doing pSB1C3 PCR with iGEM1403/1404<br><br>Conclusion: re-doing pSB1C3 PCR with iGEM1403/1404<br><br>-  Jeanne & Callum
+
description: 'Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in the fridge<br><br>-  Jeanne & Callum'},
{ title: 'construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ',
{ title: 'construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ',
start: new Date(2014,4,8),
start: new Date(2014,4,8),
-
description: 'Details: Gel purification: gel electrophoresis of pSB1C3 PCR <br><br>Result: Gel purification: gel electrophoresis of pSB1C3 PCR <br><br>Conclusion: Gel purification: gel electrophoresis of pSB1C3 PCR <br><br>-  Jeanne & Callum
+
description: 'Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>-  Jeanne & Callum'},
{ title: 'Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies',
{ title: 'Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies',
start: new Date(2014,4,8),
start: new Date(2014,4,8),
-
description: 'Details: Plasmid mini-Prep: to confirm pUS201 in top10 colonies<br><br>Result: Plasmid mini-Prep: to confirm pUS201 in top10 colonies<br><br>Conclusion: Plasmid mini-Prep: to confirm pUS201 in top10 colonies<br><br>-  Jeanne & Callum
+
description: 'Details: Incubated overnight to finish later<br><br>Result: Yield 5.7ng/ul, 1.61 260/280<br><br>Conclusion: Yield too low to use<br><br>-  Jeanne & Callum'},
{ title: 'Checking IntI1 from SamR's construct, Restriction digest: SamR's pSB1C3+ARApBAD',
{ title: 'Checking IntI1 from SamR's construct, Restriction digest: SamR's pSB1C3+ARApBAD',
start: new Date(2014,7,8),
start: new Date(2014,7,8),
-
description: 'Details: Restriction digest: SamR's pSB1C3+ARApBAD<br><br>Result: Restriction digest: SamR's pSB1C3+ARApBAD<br><br>Conclusion: Restriction digest: SamR's pSB1C3+ARApBAD<br><br>-  Rokiah
+
description: 'Details: Restriction digest (EcoR1+Pst1), incubated at 37 C for 1hr<br><br>Result: Yield: 4.46 ug/uL. Stored in -20 C fridge<br><br>Conclusion: Yield much higher than estimate provided by SamR (50ng/uL). Useable<br><br>-  Rokiah'},
{ title: 'Checking pUS201 construct, Checking pUS201 with digests',
{ title: 'Checking pUS201 construct, Checking pUS201 with digests',
start: new Date(2014,11,8),
start: new Date(2014,11,8),
-
description: 'Details: Checking pUS201 with digests<br><br>Result: Checking pUS201 with digests<br><br>Conclusion: Checking pUS201 with digests<br><br>-  ?
+
description: 'Details: Restriction Digest (uncut, EcoR1, HindIII, HindIII+BamHI)<br><br>Result: gel????? Results???<br><br>-  ?'},
{ title: 'Checking IntI1 from SamR's construct, Checking: SamR's digests on gel electrohoresis',
{ title: 'Checking IntI1 from SamR's construct, Checking: SamR's digests on gel electrohoresis',
start: new Date(2014,11,8),
start: new Date(2014,11,8),
-
description: 'Details: Checking: SamR's digests on gel electrohoresis<br><br>Result: Checking: SamR's digests on gel electrohoresis<br><br>Conclusion: Checking: SamR's digests on gel electrohoresis<br><br>-  Rokiah
+
description: 'Details: Gel electrophoresis (Spe1, uncut) at 120V for 1hr. Stained in gel red for ~30min.<br><br>Result: [12.08 SamR gel]. Cut lanes travelled further than uncut, as expected.<br><br>Conclusion: NOTE:: notes at the end cannot be read<br><br>-  Rokiah'},
{ title: 'Making IntI1 for pUS203, Restriction Digest: extracting SamR's AraC-pBAD from pSB1C3',
{ title: 'Making IntI1 for pUS203, Restriction Digest: extracting SamR's AraC-pBAD from pSB1C3',
start: new Date(2014,12,8),
start: new Date(2014,12,8),
-
description: 'Details: Restriction Digest: extracting SamR's AraC-pBAD from pSB1C3<br><br>Result: Restriction Digest: extracting SamR's AraC-pBAD from pSB1C3<br><br>Conclusion: Restriction Digest: extracting SamR's AraC-pBAD from pSB1C3<br><br>-  andy
+
description: 'Details: pSB1C3 with AraC-pBAD was incubated with *specific enzyme* at 80 C for 20 min. The enzyme was heat-killed<br><br>-  andy'},
{ title: 'Construction of pUS204, Restrction digestion of aeBlue gBlock',
{ title: 'Construction of pUS204, Restrction digestion of aeBlue gBlock',
start: new Date(2014,13,8),
start: new Date(2014,13,8),
-
description: 'Details: Restrction digestion of aeBlue gBlock<br><br>Result: Restrction digestion of aeBlue gBlock<br><br>Conclusion: Restrction digestion of aeBlue gBlock<br><br>-  abi and tom
+
description: 'Details: Restriction Digestion (EcoR1+Pst1)<br><br>Result: Yield: 1.6ng/uL<br><br>Conclusion: Enough yield to continue with ligation<br><br>-  abi and tom'},
{ title: 'Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies',
{ title: 'Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies',
start: new Date(2014,20,8),
start: new Date(2014,20,8),
-
description: 'Details: Plasmid mini-Prep: to confirm pUS201 in top10 colonies<br><br>Result: Plasmid mini-Prep: to confirm pUS201 in top10 colonies<br><br>Conclusion: Plasmid mini-Prep: to confirm pUS201 in top10 colonies<br><br>-  Callum
+
description: 'Details: Plasmid prep<br><br>Result: Yield: 3680.1ng/uL. 2.03 A260/280<br><br>Conclusion: A260/280 high ratio, may have contamination<br><br>-  Callum'},
{ title: 'Construction of pUS204, Ligation of pSB1C3 and aeBlue gBlock digestion products',
{ title: 'Construction of pUS204, Ligation of pSB1C3 and aeBlue gBlock digestion products',
start: new Date(2014,27,8),
start: new Date(2014,27,8),
-
description: 'Details: Ligation of pSB1C3 and aeBlue gBlock digestion products<br><br>Result: Ligation of pSB1C3 and aeBlue gBlock digestion products<br><br>Conclusion: Ligation of pSB1C3 and aeBlue gBlock digestion products<br><br>-  Tom
+
description: 'Details: Ligation using T4 ligase and products. Incubated for 1 hr at room temperature and them left overnight in 4C. Enzymes heat-killed at 65C.<br><br>-  Tom'},
{ title: 'Construction of pUS204, Transformaiton of ligated products into Top10 cells',
{ title: 'Construction of pUS204, Transformaiton of ligated products into Top10 cells',
start: new Date(2014,0,9),
start: new Date(2014,0,9),
-
description: 'Details: Transformaiton of ligated products into Top10 cells<br><br>Result: Transformaiton of ligated products into Top10 cells<br><br>Conclusion: Transformaiton of ligated products into Top10 cells<br><br>-  Abi and Tom
+
description: 'Details: Transformation and then incubated overnight at 37 C<br><br>-  Abi and Tom'},
{ title: 'Checking pUS201 construct, Re-checking the pUS201 by restriction digestion',
{ title: 'Checking pUS201 construct, Re-checking the pUS201 by restriction digestion',
start: new Date(2014,3,9),
start: new Date(2014,3,9),
-
description: 'Details: Re-checking the pUS201 by restriction digestion<br><br>Result: Re-checking the pUS201 by restriction digestion<br><br>Conclusion: Re-checking the pUS201 by restriction digestion<br><br>- 
+
description: 'Details: Restriction digestion (EcoR1, NdeI, NdeI/BamHI, NdeI/HindIII)<br><br>'},
{ title: 'construction of PUS204, Junction PCR to check whether pUS204 has been ligated successfully',
{ title: 'construction of PUS204, Junction PCR to check whether pUS204 has been ligated successfully',
start: new Date(2014,8,9),
start: new Date(2014,8,9),
-
description: 'Details: Junction PCR to check whether pUS204 has been ligated successfully<br><br>Result: Junction PCR to check whether pUS204 has been ligated successfully<br><br>Conclusion: Junction PCR to check whether pUS204 has been ligated successfully<br><br>-  Tom
+
description: 'Details: Junction PCR with iGEM1407/iGEM1408 primers and Taq<br><br>
-
{ title: 'Construction of pUS203, Checking: heat-killing and running a gel of products',
+
-
start: new Date(2014,9,9),
+
-
description: 'Details: Checking: heat-killing and running a gel of products<br><br>Result: Checking: heat-killing and running a gel of products<br><br>Conclusion: Checking: heat-killing and running a gel of products<br><br>-  Tom
+
-
{ title: 'Construction of pUS201, Plating out Pus201 Gibson Assembly',
+
-
start: new Date(2014,9,9),
+
-
description: 'Details: Plating out Pus201 Gibson Assembly<br><br>Result: Plating out Pus201 Gibson Assembly<br><br>Conclusion: Plating out Pus201 Gibson Assembly<br><br>-  Tom
+
-
{ title: 'Construction of pUS204, Junction PCR gel',
+
-
start: new Date(2014,9,9),
+
-
description: 'Details: Junction PCR gel<br><br>Result: Junction PCR gel<br><br>Conclusion: Junction PCR gel<br><br>- 
+
-
{ title: 'Checkin pUS203, Patch plate Int1I-2 and SamR construct',
+
-
start: new Date(2014,10,9),
+
-
description: 'Details: Patch plate Int1I-2 and SamR construct<br><br>Result: Patch plate Int1I-2 and SamR construct<br><br>Conclusion: Patch plate Int1I-2 and SamR construct<br><br>-  ?
+
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{ title: 'Checking Pus203, Preperation of colony PCR of PUS203: of the "small" and "large" colonies on patch plate',
+
-
start: new Date(2014,11,9),
+
-
description: 'Details: Preperation of colony PCR of PUS203: of the "small" and "large" colonies on patch plate<br><br>Result: Preperation of colony PCR of PUS203: of the "small" and "large" colonies on patch plate<br><br>Conclusion: Preperation of colony PCR of PUS203: of the "small" and "large" colonies on patch plate<br><br>- 
+
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{ title: 'Checking Pus203, colony PCR of PUS203: of the "small" and "large" colonies on patch plate',
+
-
start: new Date(2014,14,9),
+
-
description: 'Details: colony PCR of PUS203: of the "small" and "large" colonies on patch plate<br><br>Result: colony PCR of PUS203: of the "small" and "large" colonies on patch plate<br><br>Conclusion: colony PCR of PUS203: of the "small" and "large" colonies on patch plate<br><br>-  rokiah
+
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]     
+
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});
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});
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</script>
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</html>
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Revision as of 04:13, 14 October 2014