Team:Colombia/How to parts

From 2014.igem.org

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| style="text-align: left;" |RV pqrr4
| style="text-align: left;" |RV pqrr4
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| style="text-align: left;" |GGT CAC TAG TCA ATC TAA TAA ATG CAG ATT
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| style="text-align: left;" |GGT CAC TAG TCA ATC TAA TAA ATG CAG ATT
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| style="text-align: left;" |FW LuxOD47E
| style="text-align: left;" |FW LuxOD47E
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| style="text-align: left;" |CAA TTC TAG AAA AGA GGA GAA ATA CTA GAT G
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| style="text-align: left;" |CAA TTC TAG AAA AGA GGA GAA ATA CTA GAT G
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| style="text-align: left;" |RV LuxOD47E
| style="text-align: left;" |RV LuxOD47E
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| style="text-align: left;" |ATA TAC TAG TTG TGA CTC TAG TAG AGA
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| style="text-align: left;" |ATA TAC TAG TTG TGA CTC TAG TAG AGA
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| style="text-align: left;" |[http://parts.igem.org/Part:BBa_K581011 K581011]
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| style="text-align: left;" |[http://parts.igem.org/Part:BBa_K581011 BBa_K581011]
| style="text-align: left;" |A generator of CAI-1 behind pBAD promoter
| style="text-align: left;" |A generator of CAI-1 behind pBAD promoter
| style="text-align: left;" |Peking_Signaling 2011
| style="text-align: left;" |Peking_Signaling 2011
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|-  
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| style="text-align: left;" |[http://parts.igem.org/Part:BBa_K581009 K581009]
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| style="text-align: left;" |[http://parts.igem.org/Part:BBa_K581009 BBa_K581009]
| style="text-align: left;" |GFP behind promoter qrr
| style="text-align: left;" |GFP behind promoter qrr
| style="text-align: left;" |Peking_Signaling 2011
| style="text-align: left;" |Peking_Signaling 2011
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|-
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| style="text-align: left;" |[http://parts.igem.org/Part:BBa_K218017 K218017]
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| style="text-align: left;" |[http://parts.igem.org/Part:BBa_K218017 BBa_K218017]
| style="text-align: left;" |LuxO D47E under constitutive expression of TetR repressible promoter
| style="text-align: left;" |LuxO D47E under constitutive expression of TetR repressible promoter
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| style="text-align: left;" |Calgary 2009
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| style="text-align: left;" |Calgary 2009
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Revision as of 01:39, 26 June 2014

How to parts


Primers used to construct XXXXXXX
Name Sequence
FW pqrr4 CTT GTC TAG ATT GCA TTT TGC AAA TTT TG
RV pqrr4 GGT CAC TAG TCA ATC TAA TAA ATG CAG ATT
FW LuxOD47E CAA TTC TAG AAA AGA GGA GAA ATA CTA GAT G
RV LuxOD47E ATA TAC TAG TTG TGA CTC TAG TAG AGA



BioBricks used to construct XXXXXXX
Name Description Team
[http://parts.igem.org/Part:BBa_K581010 BBa_K581010] Regulatory proteins for receiving signaling of CAI-1 Peking_Signaling 2011
[http://parts.igem.org/Part:BBa_K581011 BBa_K581011] A generator of CAI-1 behind pBAD promoter Peking_Signaling 2011
[http://parts.igem.org/Part:BBa_K581009 BBa_K581009] GFP behind promoter qrr Peking_Signaling 2011
[http://parts.igem.org/Part:BBa_K218017 BBa_K218017] LuxO D47E under constitutive expression of TetR repressible promoter Calgary 2009

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