From 2014.igem.org
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| - | {{CSS/Delft2014_main}}
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| - | {{:Team:TU_Delft-Leiden/Templates/Start}}
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| - | <!--PAGE CONTENT----------------->
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| - | <h2> Curli Module - Cloning - Golden Gate Assembly</h2>
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| - | <p>
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| - | To construct our BioBricks <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a> three pieces of DNA had to be cloned into pSB1C3 . Both BioBricks encode a rhamnose promoter and the coding sequences of <i>csgB</i> and <i>csgA</i> or <i>csgA-HIS</i>. To work efficiently, we used the Golden Gate Assembly to construct our BioBricks. In this way we were able to restrict and ligate all the DNA fragments at once (see figure 1).
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| - | <figure>
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| - | <center><img style="margin-left: -10%;" src="https://static.igem.org/mediawiki/2014/c/c6/TUDELFT2014_goldengateCC.PNG" width="120%"> </center>
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| - | <figcaption>
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| - | Figure 1. <b>Golden Gate Assembly of <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a>.</b> The BioBricks both encode a rhamnose promoter (red rectangle) and the coding sequences of <i>csgB</i> and <i>csgA</i> or <i>csgA-HIS</i>, indicated with grey arrows. The beam beneath the arrows visualizes the regions and relative sizes of the 3 DNA pieces that were ligated into pSB1C3.
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| - | </figcaption>
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| - | {{:Team:TU_Delft-Leiden/Templates/End}}
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Latest revision as of 21:40, 13 October 2014
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