From 2014.igem.org
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| - | {{CSS/Delft2014_main}}
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| - | {{:Team:TU_Delft-Leiden/Templates/Start}}
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| - | <!--PAGE CONTENT----------------->
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| - | <h2> Curli Module - Cloning - Golden Gate Assembly</h2>
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| - | <p>
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| - | To construct the BioBricks <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a>, both encoding a rhamnose promoter and the coding sequences of <i>csgB</i> and <i>csgA</i> or <i>csgA-HIS</i>, 3 pieces of DNA had to be cloned into pSB1C3. To work efficiently, we used the LINK Golden Gate Assembly to construct our BioBricks, which made it possible to clone these BioBricks in one step only (see figure 1).
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| - | <figure>
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| - | <center><img style="margin-left: -10%;" src="https://static.igem.org/mediawiki/2014/c/c6/TUDELFT2014_goldengateCC.PNG" width="120%"> </center>
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| - | <figcaption>
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| - | Figure 1. <b>Golden Gate Assembly of <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a>.</b> The BioBricks both encode a rhamnose promoter (red rectangle) and the coding sequences of <i>csgB</i> and <i>csgA</i> or <i>csgA-HIS</i>, indicated with grey arrows. The beam beneath the arrows visualizes the regions and relative sizes of the 3 DNA pieces that were ligated into pSB1C3.
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| - | </figcaption>
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| - | </figure>
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| - | {{:Team:TU_Delft-Leiden/Templates/End}}
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Latest revision as of 21:40, 13 October 2014
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