From 2014.igem.org
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| - | {{CSS/Delft2014_main}}
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| - | {{:Team:TU_Delft-Leiden/Templates/Start}}
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| - | <!--PAGE CONTENT----------------->
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| - | <h2> Curli Module - Cloning - Golden Gate Assembly</h2>
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| - | <p>
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| - | To construct the BioBricks <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a>, both encoding a rhamnose promoter and the coding sequences of <i>csgB</i> and <i>csgA</i> or <i>csgA-HIS</i>, 3 pieces of DNA had to be cloned into pSB1C3. To work efficiently, we used the LINK Golden Gate Assembly to construct our BioBricks (see figure 1).
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| - | <figure>
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| - | <center><img style="margin-left: -10%;" src="https://static.igem.org/mediawiki/2014/c/c6/TUDELFT2014_goldengateCC.PNG" width="120%"> </center>
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| - | <figcaption>
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| - | Figure 1. <b>Golden Gate Assembly of <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a>.</b> The BioBricks both encode a rhamnose promoter (red rectangle) and the coding sequences of <i>csgB</i> and <i>csgA</i> or <i>csgA-HIS</i>, indicated with grey arrows. The beam beneath the arrows visualizes the regions and relative sizes of the 3 DNA pieces that were ligated into pSB1C3.
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| - | </figcaption>
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| - | {{:Team:TU_Delft-Leiden/Templates/End}}
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Latest revision as of 21:40, 13 October 2014
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