Team:UMaryland/Team

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Category: <a href="/OVERVIEW/2-latest">Latest </a> </span>
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<time datetime="2014-09-23T17:32:09+00:00">
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Published on 23 September 2014 </time>
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Written by <span>Super User</span> </span>
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Hits: 19 </span>
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<p style="margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: center;" dir="ltr"><em><span style="text-decoration: underline;"><strong><span style="vertical-align: baseline;"><span style="color: #000000; font-family: Arial;"><span style="font-size: 15.5555562973022px; line-height: 19.1666679382324px; white-space: pre-wrap;">Overview</span></span></span></strong></span></em></p>
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<p style="margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: left;" dir="ltr"><span style="vertical-align: baseline;"><span style="color: #000000; font-family: Arial;"><span style="font-size: 15.5555562973022px; line-height: 19.1666679382324px; white-space: pre-wrap;">The UMaryland iGem team opted for a project that hits close to home; with the Chesapeake Bay in our backyard, we have the potential to make a direct impact on the community. As Maryland’s most important natural landmark, the Chesapeake Bay is an integral part of our state’s identity. The bay is home to a diverse ecosystem, and is an economic boon to the area, supporting both the seafood industry and local tourism. Since the health of the bay is important, we looked into current problems in need of attention. In particular, the predicament of the Chesapeake’s oysters piqued our interest. As oyster populations declined over the years, the absence of this filtration system left a host of problems, including decreased water clarity and rising chemical pollutant levels. If the oyster populations can be maintained, it will improve the overall health of the Chesapeake. One way to combat oyster fatalities is to develop a way to monitor Dermo infestations. </span></span> </span></p>
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<p style="text-align: left;"><span style="font-size: 15px; font-family: Arial; color: #000000; vertical-align: baseline; white-space: pre-wrap;"> </span></p>
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<p style="line-height: 1.15; margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: center;" dir="ltr"> </p>
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<p style="line-height: 1.15; margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: center;" dir="ltr"><em><strong><span style="text-decoration: underline;"><span style="font-size: 15px; font-family: Arial; color: #000000; font-variant: normal; text-decoration: underline; vertical-align: baseline; white-space: pre-wrap; background-color: #ffffff;">Applications in the lab</span></span></strong></em></p>
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<p style="line-height: 1.15; margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: justify;" dir="ltr"><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">It is believed that </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">P. marinus </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">enters oyster hemocytes utilizing a galectin on the cell’s surface, CvGal1, that allows binds preferentially to </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">P. marinus</span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">. Our aim is to exploit CvGal1’s ability to bind to </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">P. marinus</span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;"> in order to design a biosensor for the parasite. By expressing the galectin on the surface of </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">E. coli</span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;"> and triggering a signal transduction cascade within the bacterial cell, the </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">E. coli</span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;"> could produce Green Fluorescent Protein. Thus, </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">P. marinus </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">binding to CvGal1 would trigger the expression of GFP within </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">E. coli, </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;"> creating a visual marker for the parasite. The majority of this project will be accomplished through genetic cloning and bacterial transformation. Although laboratory methods for Dermo detection using Polymerase Chain Reaction  exist, they are currently not capable of detecting the disease in real time. In the time needed to conduct a laboratory test, Dermo could have easily spread to new locations. This biosensor would help to accurately pinpoint Dermo infestation levels in real time. If we can pinpoint the location of Dermo, as elucidated in this research proposal, then we can ultimately develop a biosensor that can react to Dermo with targeted destruction. </span></p>
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<p style="line-height: 1.15; margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: justify;" dir="ltr"><span style="font-size: 15px; font-family: Arial; color: #000000; vertical-align: baseline; white-space: pre-wrap;"><strong id="docs-internal-guid-809a97bc-f5c8-6033-4262-354a17c98c1d" style="font-weight: normal;"><br /><br /></strong></span></p>
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<p style="line-height: 1.15; margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: center;" dir="ltr"><span style="text-decoration: underline;"><strong><em><span style="font-size: 15px; font-family: Arial; color: #000000; font-variant: normal; text-decoration: underline; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">Brief overview of servicing the community</span></em></strong></span></p>
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<p style="line-height: 1.15; margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: justify;" dir="ltr"><span style="font-size: 15px; font-family: Arial; color: #000000; vertical-align: baseline; white-space: pre-wrap;">We having been traveling around Maryland conducting various community outreach efforts by visiting local schools and oyster farms. During each visit, members present our project and spread awareness on the current state of the Chesapeake Bay, as well as ways individuals can help service the Bay. Some presentations are made on synthetic biology to increase student participation and interest in the sciences. We also have a committee dedicated to creating an educational computer game that teaches others about the issues that the Chesapeake Bay currently faces. It is our team’s hope that our research results to be presented at the iGEM jamboree will have a profound and positive impact on the health of the Chesapeake Bay and oyster populations worldwide</span></p>
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<li class="item-101 lifirst "><a class="afirst" href="/iGemWiki/" >Homepage</a></li><li class="item-104"><a href="/iGemWiki/index.php/grids-preview" >Photo Album</a></li><li class="item-105 parent"><a href="/iGemWiki/index.php/teaminfo" >Team Info</a><ul class="yjsgacc-dropdown" id="modid19-menu105"><li class="item-293"><a href="/iGemWiki/index.php/teaminfo/meet-the-team-members" >Team Members</a></li><li class="item-116"><a href="https://igem.org/Team.cgi?id=1489" >iGEM Page</a></li><li class="item-117"><a href="/iGemWiki/index.php/teaminfo/attributions" >Attributions</a></li><li class="item-300"><a href="/iGemWiki/index.php/teaminfo/sponsors" >Sponsors</a></li></ul></li><li class="item-112 active  parent"><a class="yjscroll" href="#" >Project</a><ul class="yjsgacc-dropdown" id="modid19-menu112"><li class="item-138 current-side  active "><a class=" isactivea" href="/OVERVIEW" >Overview</a></li><li class="item-145"><a href="/iGemWiki/index.php/features/background-motivation" >Background</a></li><li class="item-144"><a href="/iGemWiki/index.php/features/biobricks" >parts (BioBricks)</a></li><li class="item-102"><a href="/iGemWiki/index.php/features/results" target="_blank" >Results</a></li><li class="item-254"><a href="/iGemWiki/index.php/features/futureapp" >future applications</a></li><li class="item-301"><a href="/iGemWiki/index.php/features/safety" >Safety</a></li></ul></li><li class="item-234 parent"><a href="#" >Notebook</a><ul class="yjsgacc-dropdown" id="modid19-menu234"><li class="item-303"><a href="/iGemWiki/index.php/notes/wetlab" >Wetlab Notebook</a></li><li class="item-294"><a href="/iGemWiki/index.php/notes/protocols" target="_blank" >Protocols</a></li></ul></li><li class="item-123 parent"><a href="#" >Human Practices</a><ul class="yjsgacc-dropdown" id="modid19-menu123"><li class="item-106"><a href="/iGemWiki/index.php/humanpractices/overview" >Overview | Mission Statement</a></li><li class="item-129"><a href="/iGemWiki/index.php/humanpractices/outreach" >Public School Outreach</a></li><li class="item-131"><a href="/iGemWiki/index.php/humanpractices/community" >Community Interaction</a></li><li class="item-132 lilast"><a class="alast" href="/iGemWiki/index.php/humanpractices/publicawareness" >Public Awareness</a></li></ul></li></ul></div></div>
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<div class="yjsg7_before"><div id="yjsg7" class="yjsg_grid yjsgsitew"><div id="user23" class="yjsgxhtml only_mod"><div class="yjsquare modid117"><div class="h2_holder"><h2 class="module_title"><span class="title_split titlesplit0">About</span> <span class="title_split titlesplit1">Umaryland</span></h2></div><div class="yjsquare_in"><p>UMaryland2014 is University of Maryland, College Parks, inaugural iGEM team. We are a combined effort of several departments and numerous faculty mentors. Although it is only our first year, believe our hard work and dedication has paid off. We can't wait for this years competition! GO TERPS!</p></div></div></div></div></div> <div class="footer_holders footer"><!-- footer -->
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<div class="validators"><a href="http://jigsaw.w3.org/css-validator/check/referer?profile=css3" target="_blank" title="CSS Validity">CSS Valid</a> | <a href="http://validator.w3.org/check/referer" target="_blank" title="XHTML Validity">XHTML Valid</a> | <a class="yjscroll" href="#stylef6">Top</a> | <a id="fontSizePlus" class="fs" href="javascript:;" rel="nofollow">+</a> | <a id="fontSizeMinus"  class="fs" href="javascript:;" rel="nofollow">-</a> | <a id="fontSizeResetclass="fs" href="javascript:;" rel="nofollow">reset</a> | <a class="tdir" href="http://localhost/OVERVIEW?change_direction=1" rel="nofollow">RTL</a> | <a class="tdir" href="http://localhost/OVERVIEW?change_direction=2" rel="nofollow">LTR</a></div><div class="yjsgcp">Copyright &copy; <span>Baseline</span> 2014 All rights reserved. <a href="http://www.youjoomla.com" title="Joomla Templates Club">Custom Design by Youjoomla.com </a></div>       </div>
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<script type="text/javascript"> var logo_w = '240'; var site_w = '1200'; var site_f = '13px'; var sp='http://localhost/iGemWiki/'; var tp ='baseline'; var compileme =0; var fontc ='baseline_73331408039633'; var bootstrapv='bootstrap2'; var yver='2'; var yjsglegacy='1'; var yjsgrtl='2'; var menuanimation='fade';var menuanimationspeed=300; var YJSG_topmenu_font = '13px'; (function($){ $(window).load(function(){ $('.horiznav').SmoothDropJQ({ contpoz:0, horizLeftOffset: 20, horizRightOffset: -20, horizTopOffset: 20, verticalTopOffset:30, verticalLeftOffset: 10, maxOutside: 50 }); }) })(jQuery); </script>
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<a href="https://2014.igem.org/Team:UMaryland/Attributions"style="color:#000000"> Attributions </a></td>
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            <option value="/iGemWiki/">&nbsp;Homepage</option>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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            <option value="/iGemWiki/index.php/teaminfo/meet-the-team-members">&nbsp;--Meet Our Team Members</option>
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            <option value="https://igem.org/Team.cgi?id=1489">&nbsp;--iGEM Page</option>
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            <option value="/iGemWiki/index.php/teaminfo/attributions">&nbsp;--Attributions</option>
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            <option value="/iGemWiki/index.php/teaminfo/sponsors">&nbsp;--Sponsors</option>
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            <option value="#">&nbsp;Project</option>
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            <option value="/OVERVIEW" selected="selected">&nbsp;--Overview</option>
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            <option value="/iGemWiki/index.php/features/background-motivation">&nbsp;--Background</option>
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            <option value="/iGemWiki/index.php/features/biobricks">&nbsp;--parts (BioBricks)</option>
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            <option value="/iGemWiki/index.php/features/results">&nbsp;--Results</option>
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            <option value="/iGemWiki/index.php/features/futureapp">&nbsp;--future applications</option>
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            <option value="/iGemWiki/index.php/features/safety">&nbsp;--Safety</option>
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            <option value="#">&nbsp;Notebook</option>
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            <option value="/iGemWiki/index.php/notes/wetlab">&nbsp;--Wetlab Notebook</option>
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<tr><td > <h3> Team Information</h3></td>
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            <option value="/iGemWiki/index.php/notes/protocols">&nbsp;--Protocols</option>
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<td ></td >
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            <option value="#">&nbsp;Human Practices</option>
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<td > <h3> Upload pictures of your team!</h3></td>
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            <option value="/iGemWiki/index.php/humanpractices/overview">&nbsp;--Overview | Mission Statement</option>
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            <option value="/iGemWiki/index.php/humanpractices/outreach">&nbsp;--Public School Outreach</option>
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You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.</p>
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<p>
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You can look at what other teams did to get some inspiration! Here are a few examples:</p>
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<li><a href="https://2013.igem.org/Team:Evry/Team">2013 Evry</a></li>
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<li><a href="https://2013.igem.org/Team:Cornell/team">2013 Cornell</a></li>
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<li><a href="https://2013.igem.org/Team:UANL_Mty-Mexico/Team">2013 UANL Mty-Mexico</a></li>
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<li><a href="https://2013.igem.org/Team:Korea_U_Seoul/Team/Korea_University">2013 Korea U Seoul</a></li>
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<p> We provide a server where you can upload images for your wiki!</p>
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<p> Add pictures of your team members, advisors, instructors, lab, institution and everything you wish to show! </p>
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<a href="https://2014.igem.org/Special:Upload"><img src="" width="100px" height="100px"> </a> <a href="https://2014.igem.org/Special:Upload"><img src="" width="100px" height="100px"> </a>
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</a> <a href="https://2014.igem.org/Special:Upload"><img src="" width="100px" height="100px"> </a>
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<br>
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<a href="https://2014.igem.org/Special:Upload">Click here to upload! </a>
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<p>What should this page contain?</p>
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<li> You can add a small biography or a few words from each team member, tell us what you like, and what motivates to participate in iGEM. </li>
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<li>Take team pictures! Show us your school, your lab and little bit of your city.</li>
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<tr><td > <h3> Meet The Team! </h3></td>
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<p>Undergraduates</p>
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<li> Kevin Pineault (President), Junior, Bioengineering </li>
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<blockquote>I was attracted to iGEM's innovative approach of inspiring undergraduate students to lead and conduct exciting research in the growing field of synthetic biology. As a student and team member, I joined out of interest to learn about laboratory procedure to create BioBricks, to work towards a humanitarian goal alongside our research efforts, and to develop an awesome research project to combat the decreasing numbers of oysters in our local Chesapeake Bay. My role in the team has been outside the lab, developing/leading meetings and helping every student member work towards there highest potential. I have to give most of the credit the other members on the team for their '''great''' work this summer in the lab and outside the lab visiting oyster farms. I like to slackline when it is nice outside, and I am not afraid to dance in public. </blockquote>
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<li>Iowis Zhu (Vice President), Sophomore, Biochemistry / Cell Biology and Molecular Genetics Double Major (Statistics Minor)</li>
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<blockquote>Planning on conducting medical research in the future after going to medical/graduate school. I'm doing iGEM due my desire to create a concrete product that can have an impact on our world. I once memorized the periodic table to commemorate my 8th grade science teacher's retirement, and I haven't forgotten it. </blockquote>
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<li>Nathan Barber, Junior, Bioengineering </li>
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<blockquote>Pursuing a graduate degree, then a career in biomedical engineering hopefully management.
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I'm doing iGem because I'm fascinated with the impact we can make on the world as only college students.
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Fun fact: I've free climbed a 30 ft cliff outdoors with no ropes or harness.</blockquote>
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<img src="https://static.igem.org/mediawiki/2014/b/b9/UMaryland_BarberPhoto.png" alt="Nathan Profile Picture">
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<li>Adip Bhargav, Sophomore, Neurobiology & Physiology </li>
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<blockquote>I plan on going to medical school after my undergraduate studies.
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I love tennis. </blockquote>
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<li>Aditya Biswas</li>
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<li>Courtney Restivo, Freshman, Bioengineering </li>
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<blockquote>Planning on going into the bioengineering industry, possibly on the marketing side of things.
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I'm participating in iGEM because I think it's a good way to extend beyond classes and discover more about the field of genetic engineering.  I am excited by the idea of creating something that impacts the community and seeing other groups' discoveries is very motivating. 
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I can juggle a soccer ball over a hundred times.</blockquote>
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<li>Sampada Koshatwar, Sophomore, Bioengineering</li>
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<blockquote>Planning on having a career in the bioengineering industry
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I am part of iGEM because I would really like the research that comes with this competition as well take the opportunity to interact with creative scientists from all over the country and be inspired by the ideas that can come from genetical engineering.
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I can juggle three arbitrary things solo and five arbitrary things with a partner. </blockquote>
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<li>Talia Sieff</li>
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<li>Janine Taira, Freshman, Neurobiology & Physiology</li>
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<blockquote>I'm planning on going to dental school after my undergraduate degree.
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I'm participating iGem because I want to have an hands-on experience in the lab, and modifying bacteria for competition seemed like an interesting and unique way to get that experience. It's super cool that in less than a year, we'll have some kind of a product to show for all of our work.</blockquote>
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<li>Camila Uechi</li>
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<li>Pavel Alexandrovich Vlasov</li>
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<li>Ben Woodward</li>
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<li>Andrew Zhao, Freshman, Bioengineering</li>
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<blockquote>Graduate or medical school - eventual career in medical research and/or development (device engineering and whatnot)
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I joined iGEM to get experience in a wet lab where our ultimate goal is to create a working product. The fact that the students are given so much autonomy over the project is also really appealing.
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One of my hobbies is yo-yoing and I know (only) a handful of tricks - they're simple but they look cool! </blockquote>
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<p>Advisors</p>
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<li>Navadeep Boruah (Graduate Advisor)</li>
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<li>Tanya Gordonov (Graduate Advisor)</li>
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<li>Ed Eisenstein</li>
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<li>Angela Jones</li>
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<li>Jason Kahn</li>
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Latest revision as of 20:50, 13 October 2014

Overview

About Umaryland

UMaryland2014 is University of Maryland, College Parks, inaugural iGEM team. We are a combined effort of several departments and numerous faculty mentors. Although it is only our first year, believe our hard work and dedication has paid off. We can't wait for this years competition! GO TERPS!