Team:UMaryland/OVERVIEW

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Overview
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Category: <a href="/OVERVIEW/2-latest">Latest </a> </span>
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<time datetime="2014-09-23T17:32:09+00:00">
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Published on 23 September 2014 </time>
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Written by <span>Super User</span> </span>
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Hits: 19 </span>
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<p style="margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: center;" dir="ltr"><em><span style="text-decoration: underline;"><strong><span style="vertical-align: baseline;"><span style="color: #000000; font-family: Arial;"><span style="font-size: 15.5555562973022px; line-height: 19.1666679382324px; white-space: pre-wrap;">Overview</span></span></span></strong></span></em></p>
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<p style="margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: left;" dir="ltr"><span style="vertical-align: baseline;"><span style="color: #000000; font-family: Arial;"><span style="font-size: 15.5555562973022px; line-height: 19.1666679382324px; white-space: pre-wrap;">The UMaryland iGem team opted for a project that hits close to home; with the Chesapeake Bay in our backyard, we have the potential to make a direct impact on the community. As Maryland’s most important natural landmark, the Chesapeake Bay is an integral part of our state’s identity. The bay is home to a diverse ecosystem, and is an economic boon to the area, supporting both the seafood industry and local tourism. Since the health of the bay is important, we looked into current problems in need of attention. In particular, the predicament of the Chesapeake’s oysters piqued our interest. As oyster populations declined over the years, the absence of this filtration system left a host of problems, including decreased water clarity and rising chemical pollutant levels. If the oyster populations can be maintained, it will improve the overall health of the Chesapeake. One way to combat oyster fatalities is to develop a way to monitor Dermo infestations. </span></span> </span></p>
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<p style="text-align: left;"><span style="font-size: 15px; font-family: Arial; color: #000000; vertical-align: baseline; white-space: pre-wrap;"> </span></p>
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<p style="line-height: 1.15; margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: center;" dir="ltr"> </p>
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<p style="line-height: 1.15; margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: center;" dir="ltr"><em><strong><span style="text-decoration: underline;"><span style="font-size: 15px; font-family: Arial; color: #000000; font-variant: normal; text-decoration: underline; vertical-align: baseline; white-space: pre-wrap; background-color: #ffffff;">Applications in the lab</span></span></strong></em></p>
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<p style="line-height: 1.15; margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: justify;" dir="ltr"><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">It is believed that </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">P. marinus </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">enters oyster hemocytes utilizing a galectin on the cell’s surface, CvGal1, that allows binds preferentially to </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">P. marinus</span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">. Our aim is to exploit CvGal1’s ability to bind to </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">P. marinus</span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;"> in order to design a biosensor for the parasite. By expressing the galectin on the surface of </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">E. coli</span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;"> and triggering a signal transduction cascade within the bacterial cell, the </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">E. coli</span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;"> could produce Green Fluorescent Protein. Thus, </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">P. marinus </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">binding to CvGal1 would trigger the expression of GFP within </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">E. coli, </span><span style="font-size: 15px; font-family: Arial; color: #000000; background-color: #ffffff; font-weight: normal; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;"> creating a visual marker for the parasite. The majority of this project will be accomplished through genetic cloning and bacterial transformation. Although laboratory methods for Dermo detection using Polymerase Chain Reaction  exist, they are currently not capable of detecting the disease in real time. In the time needed to conduct a laboratory test, Dermo could have easily spread to new locations. This biosensor would help to accurately pinpoint Dermo infestation levels in real time. If we can pinpoint the location of Dermo, as elucidated in this research proposal, then we can ultimately develop a biosensor that can react to Dermo with targeted destruction. </span></p>
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<p style="line-height: 1.15; margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: justify;" dir="ltr"><span style="font-size: 15px; font-family: Arial; color: #000000; vertical-align: baseline; white-space: pre-wrap;"><strong id="docs-internal-guid-809a97bc-f5c8-6033-4262-354a17c98c1d" style="font-weight: normal;"><br /><br /></strong></span></p>
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<p style="line-height: 1.15; margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: center;" dir="ltr"><span style="text-decoration: underline;"><strong><em><span style="font-size: 15px; font-family: Arial; color: #000000; font-variant: normal; text-decoration: underline; vertical-align: baseline; white-space: pre-wrap; background-color: transparent;">Brief overview of servicing the community</span></em></strong></span></p>
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<p style="line-height: 1.15; margin-top: 0pt; margin-bottom: 0pt; margin-right: 4pt; text-align: justify;" dir="ltr"><span style="font-size: 15px; font-family: Arial; color: #000000; vertical-align: baseline; white-space: pre-wrap;">We having been traveling around Maryland conducting various community outreach efforts by visiting local schools and oyster farms. During each visit, members present our project and spread awareness on the current state of the Chesapeake Bay, as well as ways individuals can help service the Bay. Some presentations are made on synthetic biology to increase student participation and interest in the sciences. We also have a committee dedicated to creating an educational computer game that teaches others about the issues that the Chesapeake Bay currently faces. It is our team’s hope that our research results to be presented at the iGEM jamboree will have a profound and positive impact on the health of the Chesapeake Bay and oyster populations worldwide</span></p>
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<div class="yjsg7_before"><div id="yjsg7" class="yjsg_grid yjsgsitew"><div id="user23" class="yjsgxhtml only_mod"><div class="yjsquare modid117"><div class="h2_holder"><h2 class="module_title"><span class="title_split titlesplit0">About</span> <span class="title_split titlesplit1">Umaryland</span></h2></div><div class="yjsquare_in"><p>UMaryland2014 is University of Maryland, College Parks, inaugural iGEM team. We are a combined effort of several departments and numerous faculty mentors. Although it is only our first year, believe our hard work and dedication has paid off. We can't wait for this years competition! GO TERPS!</p></div></div></div></div></div> <div class="footer_holders footer"><!-- footer -->
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            <option value="/iGemWiki/">&nbsp;Homepage</option>
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            <option value="/iGemWiki/index.php/grids-preview">&nbsp;Photo Album</option>
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            <option value="/iGemWiki/index.php/teaminfo">&nbsp;Team Info</option>
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            <option value="/iGemWiki/index.php/teaminfo/meet-the-team-members">&nbsp;--Meet Our Team Members</option>
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Latest revision as of 20:24, 13 October 2014

Overview

About Umaryland

UMaryland2014 is University of Maryland, College Parks, inaugural iGEM team. We are a combined effort of several departments and numerous faculty mentors. Although it is only our first year, believe our hard work and dedication has paid off. We can't wait for this years competition! GO TERPS!