Team:UGA-Georgia/Protocols

From 2014.igem.org

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<li>Amplified mCherry gene with negative control group and positive control group</li>
<li>Amplified mCherry gene with negative control group and positive control group</li>
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<li>Reaction Mixture (a master mix to be divided into 3 tubes; Mq water 10.2 uL X 4 = 40.8 uL, 2x Phusion polymerase HF buffer mix 12.5 uL x 4 = 50 uL, Gene 0.5 uL x 4 = 2 uL, Forward primer 0.9 uL X 4= 3.6 uL, Reverse primer 0.9 uL x 4 = 3.6 uL </li>
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<li>Reaction Mixture (a master mix to be divided into 3 tubes; Mq water 10.2 uL X 4 = 40.8 uL, 2x Phusion polymerase HF buffer mix 12.5 uL x 4 = 50 uL, Gene 0.5 uL x 4 = 2 uL, Forward primer 0.9 uL X 4= 3.6 uL, Reverse primer 0.9 uL x 4 = 3.6 uL) </li>
<li>Final Reaction Mixture = 96.4 uL with 24.1 uL in each tube</li>
<li>Final Reaction Mixture = 96.4 uL with 24.1 uL in each tube</li>
<li>Annealing temperature = 65 Celsius </li>
<li>Annealing temperature = 65 Celsius </li>

Revision as of 00:22, 13 October 2014

HOME

PROJECT

WET LABS

TEAM

PCR

  1. Amplified mCherry gene with negative control group and positive control group
  2. Reaction Mixture (a master mix to be divided into 3 tubes; Mq water 10.2 uL X 4 = 40.8 uL, 2x Phusion polymerase HF buffer mix 12.5 uL x 4 = 50 uL, Gene 0.5 uL x 4 = 2 uL, Forward primer 0.9 uL X 4= 3.6 uL, Reverse primer 0.9 uL x 4 = 3.6 uL)
  3. Final Reaction Mixture = 96.4 uL with 24.1 uL in each tube
  4. Annealing temperature = 65 Celsius
  5. Run products on a gel for verification (2.5 uL of each)

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