Team:UGA-Georgia/Protocols
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<li>Amplified mCherry gene with negative control group and positive control group</li> | <li>Amplified mCherry gene with negative control group and positive control group</li> | ||
- | <li>Reaction Mixture (a master mix to be divided into 3 tubes; | + | <li>Reaction Mixture (a master mix to be divided into 3 tubes; Mq water 10.2 uL X 4 = 40.8 uL, 2x Phusion polymerase HF buffer mix 12.5 uL x 4 = 50 uL, Gene 0.5 uL x 4 = 2 uL, Forward primer 0.9 uL X 4= 3.6 uL, Reverse primer 0.9 uL x 4 = 3.6 uL </li> |
<li>Final Reaction Mixture = 96.4 uL with 24.1 uL in each tube</li> | <li>Final Reaction Mixture = 96.4 uL with 24.1 uL in each tube</li> | ||
<li>Annealing temperature = 65 Celsius </li> | <li>Annealing temperature = 65 Celsius </li> |
Revision as of 00:22, 13 October 2014
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