Team:TCU Taiwan/Attributions

From 2014.igem.org

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               <td colspan="2"><font size="3" face="Verdana" color="#333">Bobo&nbsp;Regina&nbsp;Peiyi&nbsp;Eating</font>
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               <td colspan="2"><font size="3" face="Verdana" color="#0092C7">Bobo, Regina, Peiyi, Eating</font>
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               <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Modeling</font></td>
               <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Modeling</font></td>
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              <td height="10px" colspan="2">&nbsp;</td>
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              <td colspan="2"><font size="3" face="Verdana" color="#0092C7">House, Bobo, Chao-Di CHANG(National Chiao Tung University)</font></td>
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               <td width="50%"><font size="3" face="Verdana" color="#333"><li>Transformation ( Cas9 in pSB1C3 into DH5α)<br>
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               <td width="50%"><font size="3" face="Verdana" color="#333"><li>Infectional efficiency ratio of M13KO7<br>
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                 <li>Extract plasmid with pSB1C3  from DH5α.<br>
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                 <li> Release titer(Releasing titer of phagemid which is E.coli infected by helper phage)<br>
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                <li>Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011.<br>
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                <li>Use Enzyme digestion (<em>PstI</em> and <em>EcoRI</em>) to confirm whether pSB1C3 and pBluescript is successfully  extracted.<br>
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                <li>Purification of RFP sequence from pSB1C3 Prepare Cm. plate、LB. plate.<br>
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                <li>Ligation of RFP and pBluescript SK(-).<br>
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                 <li>Transformation clone RFP/pBluescript SK(-) to DH5α.<br>
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                <li>Extract plasmid clone RFP/pBluescript SK(-).</li></font></td>
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               <td rowspan="3">&nbsp;</td>
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<legend><font face="Trebuchet MS" size="4" color="#fff">Experiment Design</font></legend>
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                <p><font size="3" face="Verdana" color="#90B849">Ted and                  Bobo </font><font size="3" face="Verdana" color="#90B849"><br><br>
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                </font><font size="3" face="Verdana" color="#333">
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Design gRNA for AmpR.
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<legend><font face="Trebuchet MS" size="4" color="#fff">Biobricks Design</font></legend>
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                <p><font size="3" face="Verdana" color="#90B849">Ted</font><font size="3" face="Verdana" color="#90B849"><br><br>
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                </font><font size="3" face="Verdana" color="#333">
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Design the gRNA for different resistance gene.
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                </font></p>
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</fieldset></td>
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Revision as of 23:23, 12 October 2014


 
Attributions
  
 
Experiment
 
Bobo, Regina, Peiyi, Eating
 
Contributions
 
  • Transformation ( Cas9 in pSB1C3 into DH5α)
  • Extract plasmid with pSB1C3 from DH5α.
  • Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011.
  • Use Enzyme digestion (PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.
  • Purification of RFP sequence from pSB1C3 Prepare Cm. plate、LB. plate.
  • Ligation of RFP and pBluescript SK(-).
  • Transformation clone RFP/pBluescript SK(-) to DH5α.
  • Extract plasmid clone RFP/pBluescript SK(-).
    		•   
    Experiment Design

    Ted and Bobo

    Design gRNA for AmpR.

    Biobricks Design

    Ted

     Design the gRNA for different resistance gene.

       
    Modeling
     
    House, Bobo, Chao-Di CHANG(National Chiao Tung University)
     
    Contributions
     
  • Infectional efficiency ratio of M13KO7
  • Release titer(Releasing titer of phagemid which is E.coli infected by helper phage)
    		•   
       
     
     
     
     

    iGEM Team attributions page

    Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster.

    Why do we have this requirement?

    Attribution Template

    We have this requirement to help the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, just be sure to report the work your team did and the work that was done by others.

    For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is something that is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.

    A great example of complete attribution comes from the Imperial College London 2011 team (scroll down to the bottom of their team page to see attributions).

    Here are some of the fields we recommend you have on this page. If there are other areas not listed below, but applicable to your team/project, please feel free to also list them on your attributions page. Please feel free to remove any areas not applicable to your project.

    1. General Support
    2. Project support and advice
    3. Fundraising help and advice
    4. Lab support
    5. Difficult technique support
    6. Project advisor support
    7. Wiki support
    8. Presentation coaching
    9. Policy & Practices support
    10. Thanks and acknowledgements for all other people involved in helping make a successful iGEM team.
    ^


        
    Team Members Project Parts Human Pratics Modeling Safety Notebook Attributions

    Lost the way? Use it to help you if you're lost.

    ^

    Retrieved from "http://2014.igem.org/Team:TCU_Taiwan/Attributions"