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Team:Valencia UPV/Project/results/pheromone analysis
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<div align="center"><img class="img-title" alt="Pheromone_analysis" src="https://static.igem.org/mediawiki/2014/e/e2/VUPVPheromone_analysis.png"></img></div><br/> | <div align="center"><img class="img-title" alt="Pheromone_analysis" src="https://static.igem.org/mediawiki/2014/e/e2/VUPVPheromone_analysis.png"></img></div><br/> | ||
| - | <p>It has been a hard way to obtain the first results. Summarizing: the different parts needed to build the <a class="blue-bold">pheromone biosynthesis device</a> | + | <p>It has been a hard way to obtain the first results. Summarizing: the different parts needed to build the <a class="blue-bold">pheromone biosynthesis device</a> were domesticated and assembled with the help of <a class="blue-bold">GoldenBraid 2.0</a> following the <a class="blue-bold">flowchart</a> shown in the Mehtodology section. Once the constructs were obtained, they were transiently transformed by <a class="blue-bold">agroinfiltration</a> into our plant chassis, <a class="italic">N. benthamiana</a>. At this point, it was time to check if the plant was actually producing the target pheromones.</p><br/><br/> |
Revision as of 22:17, 12 October 2014
It has been a hard way to obtain the first results. Summarizing: the different parts needed to build the pheromone biosynthesis device were domesticated and assembled with the help of GoldenBraid 2.0 following the flowchart shown in the Mehtodology section. Once the constructs were obtained, they were transiently transformed by agroinfiltration into our plant chassis, N. benthamiana. At this point, it was time to check if the plant was actually producing the target pheromones.
With this objective, the agroinfiltated plants were analysed for pheromone production using HS-SPME coupled to GC-MS. After the analysis we obtained different chromatograms to prove it. This is the volatile profile of a normal Nicotiana benthamiana leaf, the control of the experiment (Figure 1):
