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Team:Brasil-SP/Notebook/CharacterizationAssemblies
From 2014.igem.org
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<!--Início Promoter BBa_K143015 --> | <!--Início Promoter BBa_K143015 --> | ||
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<h2>Promoter BBa_K143015</h2> | <h2>Promoter BBa_K143015</h2> | ||
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<p><strong>Question</strong>:What are the concentration of QteE needed to hamper the LasR induction of the promoter PlasR?</p> | <p><strong>Question</strong>:What are the concentration of QteE needed to hamper the LasR induction of the promoter PlasR?</p> | ||
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<p>This is the most difficult task of our project. Tunning the production of QteE so that we establish the correct threshold for the discretization of the Cystatin C level in serum. To attack this challenge we designed 3 circuits so that we could plot a calibration curve. In this circuits we put the transcription of the LasR and QteE under two differnt promoters, the Pveg (BBa_K823003) and PlasR (BBa_K143015).</p> | <p>This is the most difficult task of our project. Tunning the production of QteE so that we establish the correct threshold for the discretization of the Cystatin C level in serum. To attack this challenge we designed 3 circuits so that we could plot a calibration curve. In this circuits we put the transcription of the LasR and QteE under two differnt promoters, the Pveg (BBa_K823003) and PlasR (BBa_K143015).</p> | ||
</div> | </div> | ||
Revision as of 19:57, 12 October 2014
Results: After the incubation period of the transformed E. coli a large portion of the colonies were glowing green. So the promoter does work. Moreover, this biobrick works on E. coli despite the fact it was designed for B. subtilis. |
Promoter BBa_K143015
Question: How does the transcription caused by this promoter varies with the IPTG induction?
Tunning of the QteE Threshold
Question:What are the concentration of QteE needed to hamper the LasR induction of the promoter PlasR?
