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Team:Paris Saclay/Protocols
From 2014.igem.org
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#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_without_NucleoSpin®_Tissue Extraction of the Plasmid DNA from Bacteria without NucleoSpin® Plasmid] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_without_NucleoSpin®_Tissue Extraction of the Plasmid DNA from Bacteria without NucleoSpin® Plasmid] | ||
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Gel Electrophoresis] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Gel Electrophoresis] | ||
| - | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Transformation of | + | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Transformation of CaCl2 supercompetent ''E. coli'' cells] |
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_for_the_genomic_DNA_of_bacteria PCR for the genomic DNA of bacteria] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_for_the_genomic_DNA_of_bacteria PCR for the genomic DNA of bacteria] | ||
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_clean-up PCR clean-up] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_clean-up PCR clean-up] | ||
Revision as of 19:16, 12 October 2014
Protocols
Countdown
This page is under Maher's responsibility
- Deadline: 08/oct.
- Uniformisation des textes.
- Rappeler Fabio de creer la photo pour le protocol BioBrick Assembly
- Changer les enzymes du protocol
- Text explicatif des comment et pourquoi on a fait ces protocols
- Deadline: 12/oct
- Final review.
- Preparation of supercompetent E. coli cells
- Transformation of CaCl2 supercompetent E. coli cells
- Extraction of the Plasmid DNA from Bacteria by using NucleoSpin® Plasmid
- Extraction of the Plasmid DNA from Bacteria without NucleoSpin® Plasmid
- Gel Electrophoresis
- Transformation of CaCl2 supercompetent E. coli cells
- PCR for the genomic DNA of bacteria
- PCR clean-up
- PCR for bacterial culture
- Transformation of competent cells by electroporation
- BioBrick Assembly
- P1 Phages Stock
- P1 phages transduction
- Transformation of competent E. coli by CaCl2
- Cloning in pGEMTeasy
- Electro elution of DNA from agarose gel
