"
Team:TU Delft-Leiden/Project/Life science/curli/cloning/GoldenGate
From 2014.igem.org
| Line 15: | Line 15: | ||
<center><img style="margin-left: -10%;" src="https://static.igem.org/mediawiki/2014/c/c6/TUDELFT2014_goldengateCC.PNG" width="120%"> </center> | <center><img style="margin-left: -10%;" src="https://static.igem.org/mediawiki/2014/c/c6/TUDELFT2014_goldengateCC.PNG" width="120%"> </center> | ||
<figcaption> | <figcaption> | ||
| - | Figure 1. <b>Golden Gate Assembly of <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a>.</b> The BioBricks both encode a rhamnose promoter (red rectangle) and the coding sequences of <i>csgB</i> and <i>csgA</i> or <i>csgA-HIS</i>, indicated with grey arrows. The beam beneath the arrows visualizes the regions and relative sizes of the | + | Figure 1. <b>Golden Gate Assembly of <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a>.</b> The BioBricks both encode a rhamnose promoter (red rectangle) and the coding sequences of <i>csgB</i> and <i>csgA</i> or <i>csgA-HIS</i>, indicated with grey arrows. The beam beneath the arrows visualizes the regions and relative sizes of the three DNA pieces that were ligated into pSB1C3. |
</figcaption> | </figcaption> | ||
</figure> | </figure> | ||
Revision as of 18:48, 12 October 2014
Curli Module - Cloning - Golden Gate Assembly
To construct our BioBricks BBa_K1316013 and BBa_K1316014 three pieces of DNA had to be cloned into pSB1C3 . Both BioBricks encode a rhamnose promoter and the coding sequences of csgB and csgA or csgA-HIS. To work efficiently, we used the Golden Gate Assembly to construct our BioBricks. In this way we were able to restrict and ligate all the DNA fragments at once (see figure 1).
