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Team:Brasil-SP/Project/ResponseModule
From 2014.igem.org
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<h3 align="center">Response Module</h3> | <h3 align="center">Response Module</h3> | ||
| - | <p><div align="justify"> We have chosen the GFP as a reporter gene to responding our kidney sensing because the facility to found in our laboratory, where all our professors use it in the lab for their researches. We could use another one, like YFP (yellow fluorescence protein) or RFP (red florescence protein) for exemple.</p> | + | <p><div align="justify"> The reporter gene chosen for the output system was the GFP. The main reason for that choice was the simplicity in measuring its fluorescence with the fluorimeter and flow cytometry, but it can be replaced by any other reporter system. In the final development stage of the project we envision a output that do not need to be excited like the GFP does.</p> |
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| + | <!--We have chosen the GFP as a reporter gene to responding our kidney sensing because the facility to found in our laboratory, where all our professors use it in the lab for their researches. We could use another one, like YFP (yellow fluorescence protein) or RFP (red florescence protein) for exemple.</p> | ||
<p> A blood sample from a healthy person, which has low levels of circulating Cystatin C, when in contact with B. subtilis cells triggers the expression of the reporter gene GFP in the biodetector, resulting in high intensity of fluorescence signal. However, when a blood sample from a ill person, wich has high levels of circulating Cystatin C, contacts B. subtilis cells induce the minimum of fluorescence signal, or doesn’t induce the production of GFP. In this way, the intensity of the fluorescence signal show us the diagnostic of the person, if he is sick or healthy, based in a negative signal.</div></p> | <p> A blood sample from a healthy person, which has low levels of circulating Cystatin C, when in contact with B. subtilis cells triggers the expression of the reporter gene GFP in the biodetector, resulting in high intensity of fluorescence signal. However, when a blood sample from a ill person, wich has high levels of circulating Cystatin C, contacts B. subtilis cells induce the minimum of fluorescence signal, or doesn’t induce the production of GFP. In this way, the intensity of the fluorescence signal show us the diagnostic of the person, if he is sick or healthy, based in a negative signal.</div></p> | ||
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<img src="https://static.igem.org/mediawiki/2014/4/48/No_GFP.jpg" width="200" height="auto"><p><strong>Low/or no Intensity of GFP</strong></p> | <img src="https://static.igem.org/mediawiki/2014/4/48/No_GFP.jpg" width="200" height="auto"><p><strong>Low/or no Intensity of GFP</strong></p> | ||
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Revision as of 17:48, 12 October 2014
Response Module
The reporter gene chosen for the output system was the GFP. The main reason for that choice was the simplicity in measuring its fluorescence with the fluorimeter and flow cytometry, but it can be replaced by any other reporter system. In the final development stage of the project we envision a output that do not need to be excited like the GFP does.
