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Team:Brasil-SP/Project/DiagnosisModule
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Revision as of 17:27, 12 October 2014
Diagnosis Module
It is crucial for our biodetector to have the ability to discriminate between normal and abnormal Cys C, not just responding to the presence or absence of the same. The interaction of the proteins LasR and QteE is the one responsible for giving our circuit this feature. The QteE destabilize the LasR protein, removing its activity as an inducer, or in other words creating an expression barrier. In our system, the LasR production will be under control of the ComE responsive promoter, while the QteE will be under control of the lactose promoter (IPTG inducible). Having said that, we have to adjust the IPTG concentration so that only the LasR concentration originated from increased Cys C levels. Once we have figured out the appropriate IPTG amount our bacteria will be capable of generating different outputs for normal and increased Cys C levels.
