Team:HIT-Harbin/WechatPlatform

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     <div id="project" class="nav1" onmouseover="displaySubMenu(this)" onmouseout="hideSubMenu(this)">                                    <a href="https://2014.igem.org/Team:HIT-Harbin/Project" name="top"><span>Project</span></a>
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         <li><a href="https://2014.igem.org/Team:HIT-Harbin/Abstract">Abstract</a></li>
         <li><a href="https://2014.igem.org/Team:HIT-Harbin/Abstract">Abstract</a></li>
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       <h2>Design</h2>
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       <h2>Software</h2>
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                        <img src="https://static.igem.org/mediawiki/2013/c/c6/HIT-Harbin_Project_Schematic.png" alt="" style="visibility: visible;">
 
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      <h4>Experimental Group</h4>
 
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                     <p> In order to make yeast be capable of detecting dioxins in the environment, we designed the circuit above. TEF is the constitutive promoter which can activate downstream sequences to express dual domain protein, namely, lexA-DBD/mDR83-805. mDR83-805 can express dioxin receptor hsp90. And after it combining intracellular dissociative dioxin, it will pass through karyotheca and combine with cyc1 promoter to activate the expression of green fluorescent protein downstream. Then we can know by observation whether there is dioxin in the environment or not.</p>
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<h5 class="hashed"><span><cufon class="cufon cufon-canvas" alt="1.1 Overview: " style="width: 59px; height: 18px;"><cufontext>Overview </cufontext></cufon></span></h5>
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                     <p>Hitgem is a portable type of inquiry platform for biosafety in microbiological and biomedical laboratories designed for iGemers and some researchers, based on Tencent weChat.</p>
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<p>Considering the amount of dioxin in the environment is in micro level or even trace level, when the concentration of dioxin decreases, those who can successfully combine with hsp90 and then nucleic DNA will be less. Thus, we added a feedback control to amplify the signal intensity.
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<p>It has pros as follows:</p>
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<p>a)Users on mobile phone, PC, and iPad can get access to it since it is established via weChat;</p>
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<p>b)WeChat has its great amount of users so that this platform can be spread out rapidly;</p>
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<p>c)IGemers can check out the biosafety regulations about experiments anytime.</p>
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<h5 class="hashed"><span><cufon class="cufon cufon-canvas" alt="1.2 Background: " style="width: 59px; height: 18px;"><cufontext>Background </cufontext></cufon></span></h5>
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  <p>The biosafety of synthetic biology is gathering people’s attention. What’s more, it is a must for every undergraduate team to give out instruction and guidance about safety. Meanwhile, weChat is an instant messaging software developed by Tencent China, whose weChat public platform offers API ports and has over hundred million users all over the world, such that it becomes the first choice as a portable publicizing means.
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<h5 class="hashed"><span><cufon class="cufon cufon-canvas" alt="1.3 Guidance: " style="width: 59px; height: 18px;"><cufontext>Guidance </cufontext></cufon></span></h5>
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<p>1.3.1 Log in your weChat account</p>
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<img class="Guidance" width="420px" height="714px" src="https://static.igem.org/mediawiki/2014/4/42/We1.png">
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<p>1.3.2 Choose Add <em>Contacts</em>, enter <em>hitgem</em> and search</p>
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<img class="Guidance" width="420px" height="714px" src="https://static.igem.org/mediawiki/2014/a/a3/We2.png">
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<p>1.3.3 Tap <em>Follow</em> button, then you will receive a greeting and brief instruction from <strong>hitgem</strong></p>
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<img class="Guidance" width="420px" height="714px" src="https://static.igem.org/mediawiki/2014/f/fb/We3.png">
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<p>1.3.4 Type in <em>safety</em> to get the contents of biosafety handbook</p>
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<img class="Guidance" width="420px" height="714px" src="https://static.igem.org/mediawiki/2014/b/b2/We4.png">
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<p>1.3.5 Enter the corresponding number of the info you want to know, then you will get the details</p>
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<img class="Guidance" width="420px" height="714px" src="https://static.igem.org/mediawiki/2014/2/2c/We5.png">
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<p>1.3.6 If you want info about other blocks, just change the entered number</p>
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<img class="Guidance" width="420px" height="714px" src="https://static.igem.org/mediawiki/2014/b/b0/We6.png">
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      <h4>Control Group</h4>
 
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                    <p>So as to test the working state of promoter when there is no dioxin, we designed another circuit. We replaced TEF constitutive promoter by galactose induced promoter. mDR83-805 has also been modified. Its sequence of dioxin receptor was eliminated. When it is stimulated by galactose in the environment, the circuit will express the hsp90 without the ability to combine dioxin. This circuit has no integrated hsp90 which can unite with dioxin so that it will not be affected by dioxin. It is especially for testing whether LexA-DBD can still penetrate the nuclear membrane and combine with DNA and activate downstream sequences without signal of dioxin. It works as a contrast to correct the numbers.</p>
 
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Latest revision as of 15:37, 12 October 2014

Software

Overview

Hitgem is a portable type of inquiry platform for biosafety in microbiological and biomedical laboratories designed for iGemers and some researchers, based on Tencent weChat.

It has pros as follows:

a)Users on mobile phone, PC, and iPad can get access to it since it is established via weChat;

b)WeChat has its great amount of users so that this platform can be spread out rapidly;

c)IGemers can check out the biosafety regulations about experiments anytime.

Background

The biosafety of synthetic biology is gathering people’s attention. What’s more, it is a must for every undergraduate team to give out instruction and guidance about safety. Meanwhile, weChat is an instant messaging software developed by Tencent China, whose weChat public platform offers API ports and has over hundred million users all over the world, such that it becomes the first choice as a portable publicizing means.

Guidance

1.3.1 Log in your weChat account

1.3.2 Choose Add Contacts, enter hitgem and search

1.3.3 Tap Follow button, then you will receive a greeting and brief instruction from hitgem

1.3.4 Type in safety to get the contents of biosafety handbook

1.3.5 Enter the corresponding number of the info you want to know, then you will get the details

1.3.6 If you want info about other blocks, just change the entered number

Learn More

Click here and get more details about our project.

Retrieved from "http://2014.igem.org/Team:HIT-Harbin/WechatPlatform"