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Team:TU Delft-Leiden/Project/Life science/curli/cloning/GoldenGate
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<h2> Curli Module - Cloning - Golden Gate Assembly</h2> | <h2> Curli Module - Cloning - Golden Gate Assembly</h2> | ||
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| - | To construct the BioBricks <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a>, both encoding a rhamnose promoter and the coding sequences of <i>csgB</i> and <i>csgA</i> or <i>csgA-HIS</i>, 3 pieces of DNA had to be cloned into pSB1C3. To work efficiently, we used the LINK Golden Gate Assembly to construct our BioBricks (see figure 1). | + | To construct the BioBricks <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a>, both encoding a rhamnose promoter and the coding sequences of <i>csgB</i> and <i>csgA</i> or <i>csgA-HIS</i>, 3 pieces of DNA had to be cloned into pSB1C3. To work efficiently, we used the LINK Golden Gate Assembly to construct our BioBricks, which made it possible to clone these BioBricks in one step only (see figure 1). |
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Revision as of 13:22, 12 October 2014
Curli Module - Cloning - Golden Gate Assembly
To construct the BioBricks BBa_K1316013 and BBa_K1316014 , both encoding a rhamnose promoter and the coding sequences of csgB and csgA or csgA-HIS, 3 pieces of DNA had to be cloned into pSB1C3. To work efficiently, we used the LINK Golden Gate Assembly to construct our BioBricks, which made it possible to clone these BioBricks in one step only (see figure 1).
