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Team:TU Delft-Leiden/Project/Life science/curli/cloning/GoldenGate
From 2014.igem.org
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<h2> Curli Module - Cloning - Golden Gate Assembly</h2> | <h2> Curli Module - Cloning - Golden Gate Assembly</h2> | ||
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| + | To construct the BioBricks <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a>, encoding a rhamnose promoter and the coding sequences of <i>csgB</i> and <i>csgA</i> or <i>csgA-HIS</i>, we ended up having 3 pieces of DNA to be cloned into pSB1C3. To work efficiently, we used the LINK Golden Gate Assembly to construct our BioBricks (see figure 1). | ||
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<center><img style="margin-left: -10%;" src="https://static.igem.org/mediawiki/2014/c/c6/TUDELFT2014_goldengateCC.PNG" width="120%"> </center> | <center><img style="margin-left: -10%;" src="https://static.igem.org/mediawiki/2014/c/c6/TUDELFT2014_goldengateCC.PNG" width="120%"> </center> | ||
<figcaption> | <figcaption> | ||
| - | Figure 1. <b>Golden Gate Assembly of <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a>.</b> The BioBricks encode a rhamnose promoter (red rectangle) and the coding sequences of <i>csgB</i> and <i>csgA</i> or <i>csgA-HIS</i>, indicated with grey arrows. The beam beneath the arrows visualizes the regions and relative sizes of the 3 DNA pieces that were ligated into pSB1C3. | + | Figure 1. <b>Golden Gate Assembly of <a href="http://parts.igem.org/Part:BBa_K1316013">BBa_K1316013 </a> and <a href="http://parts.igem.org/Part:BBa_K1316014">BBa_K1316014 </a>.</b> The BioBricks both encode a rhamnose promoter (red rectangle) and the coding sequences of <i>csgB</i> and <i>csgA</i> or <i>csgA-HIS</i>, indicated with grey arrows. The beam beneath the arrows visualizes the regions and relative sizes of the 3 DNA pieces that were ligated into pSB1C3. |
</figcaption> | </figcaption> | ||
</figure> | </figure> | ||
| + | </p> | ||
</html> | </html> | ||
{{:Team:TU_Delft-Leiden/Templates/End}} | {{:Team:TU_Delft-Leiden/Templates/End}} | ||
Revision as of 11:30, 12 October 2014
Curli Module - Cloning - Golden Gate Assembly
To construct the BioBricks BBa_K1316013 and BBa_K1316014 , encoding a rhamnose promoter and the coding sequences of csgB and csgA or csgA-HIS, we ended up having 3 pieces of DNA to be cloned into pSB1C3. To work efficiently, we used the LINK Golden Gate Assembly to construct our BioBricks (see figure 1).
