Team:SUSTC-Shenzhen/Notebook/HeLa Cell/blasticidin screening

From 2014.igem.org

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       name=HeLa Cell |
       name=HeLa Cell |
       date=2014|
       date=2014|
-
       goal=--to test the restriction of hela cell to blasticidin}}
+
       goal=To test the restriction of HeLa cell to blasticidin}}
=2014.8.8, 10:00=
=2014.8.8, 10:00=
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===Experimental group===
===Experimental group===
A:only about ten cells in the sightseeing
A:only about ten cells in the sightseeing
 +
B:the float cell begin to increase from2.0ug/ml
B:the float cell begin to increase from2.0ug/ml
 +
C:more float cells, and certainly in the 3.4ug/ml, plenty of float cells in the 4.0ug/ml
C:more float cells, and certainly in the 3.4ug/ml, plenty of float cells in the 4.0ug/ml
 +
D:few adherence cells(except 7.0ug/ml)
D:few adherence cells(except 7.0ug/ml)
===Repeat group===
===Repeat group===
A:few adherence cells
A:few adherence cells
 +
B:same as experimental group
B:same as experimental group
 +
C:an increase of float cell
C:an increase of float cell
 +
D:almost same as C
D:almost same as C
==Description==
==Description==
0.5ug/ml very dense(100%+)
0.5ug/ml very dense(100%+)
 +
1.4ug/ml and 1.6ug/ml have more float cells
1.4ug/ml and 1.6ug/ml have more float cells
 +
Few holes, such as 1.6ug/ml have big blank area
Few holes, such as 1.6ug/ml have big blank area
=8.10, 5:20=
=8.10, 5:20=
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==Analysis==
==Analysis==
1.the medium was emptied and the mold cannot be restricted by the antibiotics.
1.the medium was emptied and the mold cannot be restricted by the antibiotics.
 +
2.maybe we did some not standard operation.
2.maybe we did some not standard operation.

Latest revision as of 11:27, 12 October 2014

Team SUSTC-Shenzhen

Notebook

Element of an endeavor

HeLa Cell

2014 To test the restriction of HeLa cell to blasticidin

2014.8.8, 10:00

Material

Blasticidin, Hela cell, 24-holes plate

Results

Experimental group:

Row 1(0.5~1.6 ug/ml) still have many cells attach to the bottom

Row 2,3(1.8~4ug/ml) can clearly see some cells fall off, and mostly are single cell

Row 4(5~9ug/ml) certainly the cells fall off multiply

Control group(0ug/ml):

there are few fallen cells, it should be normal

Repeat group:

Just see several cells become orbicular, and the fallen cells in all holes are less than experimental groups

Description

The cells grew asymmetric, more concentrated in the center but rarer in the surrounding, and the gradients are too dense to watch out for. We infer we can get the result after 2~3days

Discussion

There is situation that we add the antibiotics asymmetric, and we used a wrong way to mix up the cell with the solid medium

2014.8.8, 4:00~5:20

Results

Experimental group

8.0ug/ml,5% cells adherence,almost cells float 0.5ug/ml,90% adherence,several float 1.0ug/ml,same as left,same as left 1.2ug/ml,same as left,same as left 1.4ug/ml,same as left,floated cells increase 1.6ug/ml,same as left,same as left
1.8ug/ml,60%~70% adherence 2.0ug/ml,same as left 2.2ug/ml,50%~60% adherence 2.4ug/ml,40%~50% adherence 2.6ug/ml,same as left 2.8ug/ml,same as left
3.0ug/ml,50%~60% adherence(increase) 3.2ug/ml,same as left 3.4ug/ml,same as left 3.6ug/ml,same as left 3.8ug/ml,same as left 4.0ug/ml,same as left
5.0ug/ml,adherence(decrease) float cell(increase) 6.0ug/ml,same as left 7.0ug/ml,same as left null null null

Repeat group

Totally, the tendency is the same as the experimental group, but there are some micro differences, the percentage of adherence is higher, meanwhile, the float cells are less than another group.

Description

The result is not the same as yesterday, and the spreading of cells is not the same spreading of cells

8.10, 9:45

Result

A8.0ug/ml 0.5ug/ml 1.0ug/ml 1.2ug/ml 1.4ug/ml 1.6ug/ml
B1.8ug/ml 2.0ug/ml 2.2ug/ml 2.4ug/ml 2.6ug/ml 2.8ug/ml
C3.0ug/ml 3.2ug/ml 3.4ug/ml 3.6ug/ml 3.8ug/ml 4.0ug/ml
D5.0ug/ml6.0ug/ml 7.0ug/ml null null null

Experimental group

A:only about ten cells in the sightseeing

B:the float cell begin to increase from2.0ug/ml

C:more float cells, and certainly in the 3.4ug/ml, plenty of float cells in the 4.0ug/ml

D:few adherence cells(except 7.0ug/ml)

Repeat group

A:few adherence cells

B:same as experimental group

C:an increase of float cell

D:almost same as C

Description

0.5ug/ml very dense(100%+)

1.4ug/ml and 1.6ug/ml have more float cells

Few holes, such as 1.6ug/ml have big blank area

8.10, 5:20

Just renew the medium of cell

8.11

Simple note

The experimental group(D4, a null hole)has an infest of mold, and we abandon this plate.

Analysis

1.the medium was emptied and the mold cannot be restricted by the antibiotics.

2.maybe we did some not standard operation.

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