Team:UT-Tokyo/CTCD/Content

From 2014.igem.org

(Difference between revisions)
Line 72: Line 72:
<img src = "https://static.igem.org/mediawiki/2014/8/8f/SubCTC_system.png" class = "contTitle"/>
<img src = "https://static.igem.org/mediawiki/2014/8/8f/SubCTC_system.png" class = "contTitle"/>
<img src="https://static.igem.org/mediawiki/2014/6/64/Kobari_pegp2.png" class = "figure" />
<img src="https://static.igem.org/mediawiki/2014/6/64/Kobari_pegp2.png" class = "figure" />
 +
<legend>Fig.3 the construct of our circuit</legend>
<h3>EGP-2 promoter</h3>
<h3>EGP-2 promoter</h3>
<p>EGP-2 promoter is the promoter of Epithelial cell adhesion molecule (EpCAM) protein.</p>
<p>EGP-2 promoter is the promoter of Epithelial cell adhesion molecule (EpCAM) protein.</p>
Line 77: Line 78:
<h3>miRNA-142</h3>
<h3>miRNA-142</h3>
<p>MiRNA is a class of non-coding RNA.MiRNA binds the miRNA-recognition element in the 3' untranslated region of the target gene. MiRNA shows the tissue-specific expression pattern. In our project, we use miRNA-142, which is expressed only in hematopoietic cells[3].Adding the recognition element of miRNA-142 in the 3' untranslated region of the reporter genes, leaky expression in non-carcinoma hematopoietic cells can be reduced.</p>
<p>MiRNA is a class of non-coding RNA.MiRNA binds the miRNA-recognition element in the 3' untranslated region of the target gene. MiRNA shows the tissue-specific expression pattern. In our project, we use miRNA-142, which is expressed only in hematopoietic cells[3].Adding the recognition element of miRNA-142 in the 3' untranslated region of the reporter genes, leaky expression in non-carcinoma hematopoietic cells can be reduced.</p>
-
<img src="https://static.igem.org/mediawiki/2014/4/44/Kobari_hematopoietic_cell.png" class = "figure" />
 
<img src="https://static.igem.org/mediawiki/2014/9/94/Kobari_CTC.png" class = "figure" />
<img src="https://static.igem.org/mediawiki/2014/9/94/Kobari_CTC.png" class = "figure" />
 +
<legend>Fig.4 When this construct is transfected to the epithelial cells like cancer cell, pEGP-2 promote the transcription of EGFP, and the mRNA is not degraded by miRNA because miR 142 is specific to hematopoietic cells.</legend>
 +
<img src="https://static.igem.org/mediawiki/2014/4/44/Kobari_hematopoietic_cell.png" class = "figure" />
 +
<legend>Fig.5 When this construct is transfected to the hematopoietic cells, pEGP-2 does not work as a promoter, and mRNA is not transcribed. Even if the leak of the promoter makes few mRNA ,this mRNA is degraded by RNAi caused by miR 142.</legend>
</div>
</div>
<!-- Project application -->
<!-- Project application -->

Revision as of 09:55, 12 October 2014

Retrieved from "http://2014.igem.org/Team:UT-Tokyo/CTCD/Content"