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Team:EPF Lausanne/Microfluidics/Making/PartII
From 2014.igem.org
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<td>The PDMS mixture is precisely weighted. The ratio of organic polymer to curing agent is an important factor for a good chip consistency.</td> | <td>The PDMS mixture is precisely weighted. The ratio of organic polymer to curing agent is an important factor for a good chip consistency.</td> | ||
| - | <td class="cntr"><a href="https://static.igem.org/mediawiki/2014/c/cd/Greg1.png" data-lightbox="img1" data-title="Weighting PDMS"><img src="https://static.igem.org/mediawiki/2014/c/cd/Greg1.png" width=" | + | <td class="cntr"><a href="https://static.igem.org/mediawiki/2014/c/cd/Greg1.png" data-lightbox="img1" data-title="Weighting PDMS"><img src="https://static.igem.org/mediawiki/2014/c/cd/Greg1.png" width="80%"/></a></td> |
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Once the mixtures are ready, they need to be mixed thoroughly in a centrifugal mixer.</td> | ||
| + | <td class="cntr"><a href="https://static.igem.org/mediawiki/2014/d/d0/Greg2.png" data-lightbox="img1" data-title="Mix in the centrifugal mixer"><img src="https://static.igem.org/mediawiki/2014/d/d0/Greg2.png" width="80%"/></a></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>The PDMS mixture is precisely weighted. The ratio of organic polymer to curing agent is an important factor for a good chip consistency.</td> | ||
| + | <td class="cntr"><a href="https://static.igem.org/mediawiki/2014/c/cd/Greg1.png" data-lightbox="img1" data-title="Weighting PDMS"><img src="https://static.igem.org/mediawiki/2014/c/cd/Greg1.png" width="80%"/></a></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>The PDMS mixture is precisely weighted. The ratio of organic polymer to curing agent is an important factor for a good chip consistency.</td> | ||
| + | <td class="cntr"><a href="https://static.igem.org/mediawiki/2014/c/cd/Greg1.png" data-lightbox="img1" data-title="Weighting PDMS"><img src="https://static.igem.org/mediawiki/2014/c/cd/Greg1.png" width="80%"/></a></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>The PDMS mixture is precisely weighted. The ratio of organic polymer to curing agent is an important factor for a good chip consistency.</td> | ||
| + | <td class="cntr"><a href="https://static.igem.org/mediawiki/2014/c/cd/Greg1.png" data-lightbox="img1" data-title="Weighting PDMS"><img src="https://static.igem.org/mediawiki/2014/c/cd/Greg1.png" width="80%"/></a></td> | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
Revision as of 00:13, 12 October 2014
Chip manufacturing
The chip creation procedure is divided into several steps. These steps are summarized in the figure above. First one needs to create a mask (see c) in order to obtain a master (see d) starting from a silicon wafer (see a). For the specific procedure of mask and wafer creation, see Part I. Once the master is ready it can be used as a mold to create the PDMS chip.
Here is a non-exhaustive step by step presentation. For the precise procedure you can refer to our notebook protocol.
| The PDMS mixture is precisely weighted. The ratio of organic polymer to curing agent is an important factor for a good chip consistency. |  |
| Once the mixtures are ready, they need to be mixed thoroughly in a centrifugal mixer. |  |
| The PDMS mixture is precisely weighted. The ratio of organic polymer to curing agent is an important factor for a good chip consistency. | |
| The PDMS mixture is precisely weighted. The ratio of organic polymer to curing agent is an important factor for a good chip consistency. | |
| The PDMS mixture is precisely weighted. The ratio of organic polymer to curing agent is an important factor for a good chip consistency. | |
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