Team:Brasil-SP/Project/ResponseModule

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<h3 align="center">Response Module</h3>
<h3 align="center">Response Module</h3>
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<p><div align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;A blood sample from a healthy person, which has low levels of circulating Cystatin C, when in contact with B. subtilis cells triggers the expression of the reporter gene GFP in the biodetector, resulting in fluorescent signals. However, when a blood sample from a ill person, wich has high levels of circulating Cystatin C, contacts B. subtilis cells it doesn’t induce the production of GFP. The difference of fluorescence show us the diagnostic of the person, if he is sick or healthy, based in a negative signal.</p> 
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<p><div align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We have chosen the GFP as a reporter gene to responding our kidney sensing because the facility to found in our laboratory, we could use another one, like YFP (yellow fluorescence protein) or RFP (red florescence protein) for exemple.</p> 
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The GFP was chosen because the facility to found in our laboratory, we could use another reporter gene, like YFP (yellow fluorescence protein) or RFP (red florescence protein) for exemple.</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;A blood sample from a healthy person, which has low levels of circulating Cystatin C, when in contact with B. subtilis cells triggers the expression of the reporter gene GFP in the biodetector, resulting in high intensity of fluorescence signal. However, when a blood sample from a ill person, wich has high levels of circulating Cystatin C, contacts B. subtilis cells induce the minimus of fluorescence signal, or doesn’t induce the production of GFP. In this way, the intensity of the fluorescence signal show us the diagnostic of the person, if he is sick or healthy, based in a negative signal.</div></p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;All the blood samples will be filtered through a microfluidics device, extracting just the plasma, that will be analyzed after it contacts with our modified B. subtilis.</p>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In this device, we will store B. subtilis as spores, which are activated by rich medium (Luria-Bertani [LB]) before being used in the device. In order to induce the spore formation, the bacteria are incubated in a minimal nutrient medium (Difco Sporulation Medium [DSM]).
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Revision as of 23:02, 11 October 2014

Response Module

     We have chosen the GFP as a reporter gene to responding our kidney sensing because the facility to found in our laboratory, we could use another one, like YFP (yellow fluorescence protein) or RFP (red florescence protein) for exemple.

     A blood sample from a healthy person, which has low levels of circulating Cystatin C, when in contact with B. subtilis cells triggers the expression of the reporter gene GFP in the biodetector, resulting in high intensity of fluorescence signal. However, when a blood sample from a ill person, wich has high levels of circulating Cystatin C, contacts B. subtilis cells induce the minimus of fluorescence signal, or doesn’t induce the production of GFP. In this way, the intensity of the fluorescence signal show us the diagnostic of the person, if he is sick or healthy, based in a negative signal.

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