Team:Penn/Safety

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<p><a href="http://www.upenn.edu">University of Pennsylvania</a> <span>iGEM 2014</span></p>
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Penn/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:Penn"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:Penn/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Penn"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:Penn/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:Penn/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:Penn/Modeling"style="color:#000000"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:Penn/Notebook"style="color:#000000"> Notebook</a></td>
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<a href="https://2014.igem.org/Team:Penn/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:Penn/Attributions"style="color:#000000"> Attributions </a></td>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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<tr><td > <h3> Welcome! </h3></td>
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<td > <h3> Timeline</h3></td>
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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<h3> Edit this page!</h3>
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Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
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<li> <a href=" ">Learn about lab Safety for Today</a></li>  
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<li><a href="https://2014.igem.org/Team:Penn"><span class="menu_item">Home</span></a></li>
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<li> <a href="">Learn about Safety for the future of your project.</a></li>  
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<li><a href="https://2014.igem.org/Team:Penn/Overview" ><span class="menu_item">Project</span></a>
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<li><a href="https://2014.igem.org/Team:Penn/Overview">Overview</a></li>
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<li><a href="https://2014.igem.org/Team:Penn/Motivation">Motivation</a></li>
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<li><a href="https://2014.igem.org/Team:Penn/Protocol"><span class="menu_item">Wet lab</span></a>
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                        <li><a href="https://2014.igem.org/Team:Penn/Protocol">Protocol</a></li>
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                        <li><a href="https://2014.igem.org/Team:Penn/Safety">Safety</a></li>
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                        <li><a href="https://2014.igem.org/Team:Penn/Notebook">Notebook</a></li>
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                        <li><a href="https://2014.igem.org/Team:Penn/Outreach">Outreach</a></li>
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<li><a href="https://2014.igem.org/Team:Penn/Team">Team</a></li>
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<h3> Your Lab </h3>
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>  
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Image:Example2_Lab_1.png|The building our lab is in!
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Image:Example2_Lab_3.png|Team Member 3 doing an experiment
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Image:Example2_Lab_4.png|Working in biosafety cabinets
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Image:Example2_Lab_5.png|Team all gloved up and ready for work!
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<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
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<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
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<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
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<h2>Biological Safety</h2>
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    Our work throughout iGEM has been split between using both three different E. coli strains (DH5 alpha, W3110, and MG 1655) and Magnetospirillum Magneticum AMB-1. All four of these strains pose minimal risk inside the lab. We use strict biosafety laboratory techniques when handling these organisms and any piece of labware that comes in contact with them.
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<h3>E.coli</h3>
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<p>All three strains of E. coli are highly conserved and pose minor risks when handeled carefully in the lab. All three strains of E. coli are rod shaped, gram negative bacteria. While E. coli resides in the intestinal tract of many organisms, all three straisn used in the lab are safe to work with as they lack the adaptability and capability to cause infection. ATCC has assigned all strains a biosafety level of 1.</p>
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<h3>Magnetospirillum Magneticum AMB-1</h3>
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<p>Magnetospirillum Magneticum AMB-1 is a strain of magnetic bacteria that poses minimal health risk to humans in a lab environment. This strain of bacteria is too sensitive to environmental conditions to cause infections to humans. At the same time all procedures used to handle E. coli are used with Magnetospirillum Magneticum AMB-1 as well. This includes bleaching old cultures and disposing old tubes according to biohazard protocols.
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<h3>Public Safety</h3>
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<p>Since some of our bacteria may have antibiotic resistance and could theoretically pose a health risk, we use biohazard protocols to prevent any release of our bacteria from the lab. This includes bleaching cultures before disposing of them and disposing of any old tubes in biohazard containers. This eliminates the risk of any bacteria escaping our lab or making their way into any adjacent labs.
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<h3>Handling and disposal of Cadmium and other Inorganic Toxic Substances</h3>
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We took cautinous measures when handling cadmium and other toxic substances. <span style="font-style:italic">Lab coats, masks, gloves, and goggles</span> are worn all time. The handling is done in a fume hood to prevent inhalation of toxic substances.
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We followed protocols established by Environmental Health &amp; Radiation Safety at the University of Pennsylvania to dispose any toxic substances. We disposed the waste in designated containers and trained specialists will pick up the containers from lab and dispose of the waste properly.</p>
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Revision as of 21:20, 11 October 2014



Penn iGEM Wiki 2014














	
Biological Safety

	

     Our work throughout iGEM has been split between using both three different E. coli strains (DH5 alpha, W3110, and MG 1655) and Magnetospirillum Magneticum AMB-1. All four of these strains pose minimal risk inside the lab. We use strict biosafety laboratory techniques when handling these organisms and any piece of labware that comes in contact with them.
	

	

	

	
E.coli

	
All three strains of E. coli are highly conserved and pose minor risks when handeled carefully in the lab. All three strains of E. coli are rod shaped, gram negative bacteria. While E. coli resides in the intestinal tract of many organisms, all three straisn used in the lab are safe to work with as they lack the adaptability and capability to cause infection. ATCC has assigned all strains a biosafety level of 1.

	
Magnetospirillum Magneticum AMB-1

	
Magnetospirillum Magneticum AMB-1 is a strain of magnetic bacteria that poses minimal health risk to humans in a lab environment. This strain of bacteria is too sensitive to environmental conditions to cause infections to humans. At the same time all procedures used to handle E. coli are used with Magnetospirillum Magneticum AMB-1 as well. This includes bleaching old cultures and disposing old tubes according to biohazard protocols.
	

	
Public Safety

	
Since some of our bacteria may have antibiotic resistance and could theoretically pose a health risk, we use biohazard protocols to prevent any release of our bacteria from the lab. This includes bleaching cultures before disposing of them and disposing of any old tubes in biohazard containers. This eliminates the risk of any bacteria escaping our lab or making their way into any adjacent labs.
    

	
Handling and disposal of Cadmium and other Inorganic Toxic Substances

	

	We took cautinous measures when handling cadmium and other toxic substances. Lab coats, masks, gloves, and goggles are worn all time. The handling is done in a fume hood to prevent inhalation of toxic substances.
	

	

	We followed protocols established by Environmental Health & Radiation Safety at the University of Pennsylvania to dispose any toxic substances. We disposed the waste in designated containers and trained specialists will pick up the containers from lab and dispose of the waste properly.
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