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Team:Brasil-SP/Project/DiagnosisModule
From 2014.igem.org
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<h3 align="center">Diagnosis Module</h3> | <h3 align="center">Diagnosis Module</h3> | ||
<p><div align="justify"> If the concentration of Cystatin C is normal, the activity of Cathepsin S is not sufficiently inhibited. Thus, the linker will be cleaved, releasing AIP. The AIP activates ComD, which, in turn, phosphorylates ComE. ComE activates the LasR production, so that it achieves optimal concentration to activate the promoter, even if several LasR proteins interact with qteE. Therefore, the transcription of the reporter gene, GFP, is activated. If the concentration of Cystatin C is above normal, indicating a possible kidney disease, the activity of Cathepsin S is inhibited. In this way, there will not be cleavage of the linker nor the release of AIP. Thus the chain of events is not triggered, so there is no production of the reporter gene.</div></p> | <p><div align="justify"> If the concentration of Cystatin C is normal, the activity of Cathepsin S is not sufficiently inhibited. Thus, the linker will be cleaved, releasing AIP. The AIP activates ComD, which, in turn, phosphorylates ComE. ComE activates the LasR production, so that it achieves optimal concentration to activate the promoter, even if several LasR proteins interact with qteE. Therefore, the transcription of the reporter gene, GFP, is activated. If the concentration of Cystatin C is above normal, indicating a possible kidney disease, the activity of Cathepsin S is inhibited. In this way, there will not be cleavage of the linker nor the release of AIP. Thus the chain of events is not triggered, so there is no production of the reporter gene.</div></p> | ||
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Revision as of 21:03, 11 October 2014
Diagnosis Module
If the concentration of Cystatin C is normal, the activity of Cathepsin S is not sufficiently inhibited. Thus, the linker will be cleaved, releasing AIP. The AIP activates ComD, which, in turn, phosphorylates ComE. ComE activates the LasR production, so that it achieves optimal concentration to activate the promoter, even if several LasR proteins interact with qteE. Therefore, the transcription of the reporter gene, GFP, is activated. If the concentration of Cystatin C is above normal, indicating a possible kidney disease, the activity of Cathepsin S is inhibited. In this way, there will not be cleavage of the linker nor the release of AIP. Thus the chain of events is not triggered, so there is no production of the reporter gene.
