Team:Austin Texas/interlab study

From 2014.igem.org

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(Devices Measured)
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=Devices Measured=
=Devices Measured=
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1. [http://parts.igem.org/Part:BBa_I20260 BBa_I20260]  
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'''1.''' The first device is [http://parts.igem.org/Part:BBa_I20260 BBa_I20260], which is a biobrick part that contains:
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([http://parts.igem.org/Part:BBa_J23101 BBa_J23101] (strong constitutive promoter)
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[http://parts.igem.org/Part:BBa_E0240 BBa_E0240] ([http://parts.igem.org/Part:BBa_B0032 B0032] - RBS, [http://parts.igem.org/Part:BBa_E0040 E0040] - GFP, [http://parts.igem.org/Part:BBa_B0015 B0015] - double terminator))
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in [http://parts.igem.org/Part:pSB1C3 pSB1C3] (high copy BioBrick assembly plasmid)
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A promoter, [http://parts.igem.org/Part:BBa_J23101 BBa_J23101], a downstream gene ([http://parts.igem.org/Part:BBa_E0240 BBa_E0240]), and a backbone ([http://parts.igem.org/Part:pSB1C3 pSB1C3]).
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2. [http://parts.igem.org/Part:BBa_J23101 BBa_J23101] (strong constitutive promoter)
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The downstream gene is also a composite of a number of biobrick parts, including an RBS - [http://parts.igem.org/Part:BBa_B0032 B0032], a GFP coding sequence - [http://parts.igem.org/Part:BBa_E0040 E0040], and a transcriptional terminator - [http://parts.igem.org/Part:BBa_B0015 B0015].
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[http://parts.igem.org/Part:BBa_E0240 BBa_E0240] ([http://parts.igem.org/Part:BBa_B0032 B0032] - RBS, [http://parts.igem.org/Part:BBa_E0040 E0040] - GFP, [http://parts.igem.org/Part:BBa_B0015 B0015] - double terminator)
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in [http://parts.igem.org/Part:pSB3K3 pSB3K3] (low to medium copy BioBrick standard vector)
 
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3. [http://parts.igem.org/Part:BBa_J23115 BBa_J23115] (weak constitutive promoter)
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'''2.''' The second device was cloned in lab, as described in the interlab study procedure.
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[http://parts.igem.org/Part:BBa_E0240 BBa_E0240] ([http://parts.igem.org/Part:BBa_B0032 B0032] - RBS, [http://parts.igem.org/Part:BBa_E0040 E0040] - GFP, [http://parts.igem.org/Part:BBa_B0015 B0015] - double terminator)
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in [http://parts.igem.org/Part:pSB1C3 pSB1C3] (high copy BioBrick assembly plasmid)
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The device is composed of [http://parts.igem.org/Part:BBa_J23101 BBa_J23101] and [http://parts.igem.org/Part:BBa_E0240 BBa_E0240], which are the same parts as in the first.  However, the backbone of this device is different, [http://parts.igem.org/Part:pSB3K3 pSB3K3]
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'''3.''' The third device was also cloned in lab, as described in the interlab study procedure.
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The device is composed of [http://parts.igem.org/Part:BBa_J23115 BBa_J23115] and [http://parts.igem.org/Part:BBa_E0240 BBa_E0240], which is the same downstream gene as in devices 1 and 2.  However, the promoter sequence of device 3 is different.  This device is in [http://parts.igem.org/Part:pSB1C3 pSB1C3].
[[File:interlabliquidcultures.jpg|400px]]
[[File:interlabliquidcultures.jpg|400px]]

Revision as of 20:47, 11 October 2014