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Team:ETH Zurich/labblog/20140825meet
From 2014.igem.org
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* We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. Check <html> <a id="insidelink" target="diffusionexp" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html> for more details. | * We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. Check <html> <a id="insidelink" target="diffusionexp" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html> for more details. | ||
| - | *We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length. | + | *We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length. Check <html> <a id="insidelink" targetid="diffcomsol" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffcomsol"> this article </a> </html> for more details. |
| - | + | ||
* We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves. | * We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves. | ||
Revision as of 18:59, 11 October 2014
Week 14
Monday, August 25th
- We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. Check the corresponding article for more details.
- We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length. Check this article for more details.
- We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves.
