Team:Aix-Marseille/Safety

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<br>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Aix-Marseille/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:Aix-Marseille"style="color:#000000">Home </a> </td>
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    <h1 class="project-title">Safety issues</h1>
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    <div class="row" style="position:relative;">
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      <!-- Content -->
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      <!-- ******* -->
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        <div class="project-section">
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          <span class="project-tag" id="overall"></span>
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          <h1 style="border-bottom-color:#f39c12">Overall</h1>
 +
          <p>All laboratory work needs to follow safety rules and regulations. We manipulated in a CNRS laboratory on Joseph Aiguier's campus. That's why, we stuck to CNRS guidelines concerning genetically modified organism manipulation. All members of our iGEM team attended a conference on biosafety guidelines in CNRS laboratories.</p>
 +
        </div>
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        <div class="project-section">
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          <span class="project-tag" id="introduction"></span>
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          <h1 style="border-bottom-color:#f39c12">General introduction</h1>
 +
          <p>The manipulation of Genetically Moified Organisms is supposed to be strictly supervised and there are specific regulations. Safety issues are separated in several areas: chemicals and hasardous products, risky devices and equipment and bacteriologic risks.</p>
 +
        </div>
 +
       
 +
        <div class="project-section">
 +
          <span class="project-tag" id="chemical_risks"></span>
 +
          <h1 style="border-bottom-color:#f39c12">Chemical risks</h1>
 +
          <p>We used some hazardous products which need to be vigilant.</p>
 +
          <ul>
 +
            <li>
 +
              <h3 class="subtitle">GelRed</h3>
 +
              <p>We used GelRed as an intercalating nucleic acid strain for agarose gel electrophoresis. It needed to be manipulated with gloves because of it chemical nature.</p>
 +
            </li>
 +
            <li>
 +
              <h3 class="subtitle">Diméthylsulfoxyde</h3>
 +
              <p>DMSO was used in PCR reactions. It should be manipulated with caution because it can cause contaminants, toxins, and medicines to be absorbed through the skin.</p>
 +
            </li>
 +
            <li>
 +
              <h3 class="subtitle">Macherey Nagel MiniPrep Kit</h3>
 +
              <p>As every kit of plasmid purification, this kit could contain toxic solvents hazardous for humans and the environment. All waste produced using this kit must be correctly treated.</p>
 +
            </li>
 +
          </ul>
 +
        </div>
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 +
        <div class="project-section">
 +
          <span class="project-tag" id="physical_risks"></span>
 +
          <h1 style="border-bottom-color:#f39c12">Physical risks</h1>
 +
          <p>Some devices used during manipulation in laboratory could be risky.</p>
 +
          <ul>
 +
            <li>
 +
              <h3 class="subtitle">UV revelation machine</h3>
 +
              <p>To reveal agarose gel and to realize gel extractions, we used an UV revelation machine. UV may cause blindness if no protection masks is worn.</p>
 +
            </li>
 +
            <li>
 +
              <h3 class="subtitle">Centrifuge</h3>
 +
              <p>Used for many protocols, the rotor in centrifuges can be dangerous, it needs to be checked before each use. It may cause death in worst case scenarios or even wreck a building and amenities.</p>
 +
            </li>
 +
            <li>
 +
              <h3 class="subtitle">-80°C Freezer</h3>
 +
              <p>Special warm gloves are required to manipulate what is inside the freezer, can causes freeze burns to the skin.</p>
 +
            </li>
 +
            <li>
 +
              <h3 class="subtitle">Water bath</h3>
 +
              <p>Used in competent cells protocols. One have to be careful to turn it off when manipulation is over for it may cause fire if all water evaporates.</p>
 +
            </li>
 +
            <li>
 +
              <h3 class="subtitle">Scalpels</h3>
 +
              <p>Scalpels were used to slice agarose gels and more generally in of gel extraction protocols. Those who use it have to be careful not to cut themselves, and safely dispose of the blades.</p>
 +
            </li>
 +
          </ul>
 +
        </div>
 +
       
 +
        <div class="project-section">
 +
          <span class="project-tag" id="bacteriological_risks"></span>
 +
          <h1 style="border-bottom-color:#f39c12">Bacteriological risks</h1>
 +
          <p>All bacteria used during the project are classified risk group 1 in biosafety guidelines. We worked exclusively with Escherichia coli stains DH5α and W3110 both of which are of the K12 type, and thus not pathogenic to humans. However, there is still a small residual risk of contamination especially of eyes and the respiratory pathway.</p>
 +
          <p>As the organisms used are genetically modified, cultures and microbiological waste are autoclaved after use, thus the GMOs cannot get escape from the laboratory environment. </p>
 +
        </div>
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<a href="https://2014.igem.org/Team:Aix-Marseille/Team"style="color:#000000"> Team </a> </td>
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              <a data-scroll href="#overall">Overall</a>
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            <li>
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              <a data-scroll href="#introduction">Introduction</a>
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            </li>
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            <li>
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              <a data-scroll href="#chemical_risks">Chemical risks</a>
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              <a data-scroll href="#physical_risks">Physical risks</a>
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              <a data-scroll href="#bacteriological_risks">Bacteriological risks</a>
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{{Team:Aix-Marseille/footer}}
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<tr><td > <h3> Welcome! </h3></td>
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
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<li> <a href="">Learn about Safety for the future of your project.</a></li>
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-->
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<h3> Your Lab </h3>
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
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Image:Example2_Lab_1.png|The building our lab is in!
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Image:Example2_Lab_2.png|The inside of our lab!
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Image:Example2_Lab_3.png|Team Member 3 doing an experiment
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Image:Example2_Lab_4.png|Working in biosafety cabinets
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Image:Example2_Lab_5.png|Team all gloved up and ready for work!
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Image:Example2_Lab_6.png|Equipment that we use to do SCIENCE!
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Image:Example2_Lab_7.png|We decorated this part of our lab
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Image:Example2_Lab_8.png|Whatever else you want
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<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
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<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
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<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
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Latest revision as of 10:31, 11 October 2014

Safety issues

Overall

All laboratory work needs to follow safety rules and regulations. We manipulated in a CNRS laboratory on Joseph Aiguier's campus. That's why, we stuck to CNRS guidelines concerning genetically modified organism manipulation. All members of our iGEM team attended a conference on biosafety guidelines in CNRS laboratories.

General introduction

The manipulation of Genetically Moified Organisms is supposed to be strictly supervised and there are specific regulations. Safety issues are separated in several areas: chemicals and hasardous products, risky devices and equipment and bacteriologic risks.

Chemical risks

We used some hazardous products which need to be vigilant.

  • GelRed

    We used GelRed as an intercalating nucleic acid strain for agarose gel electrophoresis. It needed to be manipulated with gloves because of it chemical nature.

  • Diméthylsulfoxyde

    DMSO was used in PCR reactions. It should be manipulated with caution because it can cause contaminants, toxins, and medicines to be absorbed through the skin.

  • Macherey Nagel MiniPrep Kit

    As every kit of plasmid purification, this kit could contain toxic solvents hazardous for humans and the environment. All waste produced using this kit must be correctly treated.

Physical risks

Some devices used during manipulation in laboratory could be risky.

  • UV revelation machine

    To reveal agarose gel and to realize gel extractions, we used an UV revelation machine. UV may cause blindness if no protection masks is worn.

  • Centrifuge

    Used for many protocols, the rotor in centrifuges can be dangerous, it needs to be checked before each use. It may cause death in worst case scenarios or even wreck a building and amenities.

  • -80°C Freezer

    Special warm gloves are required to manipulate what is inside the freezer, can causes freeze burns to the skin.

  • Water bath

    Used in competent cells protocols. One have to be careful to turn it off when manipulation is over for it may cause fire if all water evaporates.

  • Scalpels

    Scalpels were used to slice agarose gels and more generally in of gel extraction protocols. Those who use it have to be careful not to cut themselves, and safely dispose of the blades.

Bacteriological risks

All bacteria used during the project are classified risk group 1 in biosafety guidelines. We worked exclusively with Escherichia coli stains DH5α and W3110 both of which are of the K12 type, and thus not pathogenic to humans. However, there is still a small residual risk of contamination especially of eyes and the respiratory pathway.

As the organisms used are genetically modified, cultures and microbiological waste are autoclaved after use, thus the GMOs cannot get escape from the laboratory environment.

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