Team:HIT-Harbin/Judging

From 2014.igem.org

(Difference between revisions)
Line 48: Line 48:
     </div>
     </div>
     <!--humanPractic -->
     <!--humanPractic -->
-
     <div id="humanPractic" class="nav1" onmouseover="displaySubMenu(this)" onmouseout="hideSubMenu(this)"><a href="https://2014.igem.org/Team:HIT-Harbin/Humanpractic">Human&nbsp;&nbsp;practic</a>
+
     <div id="humanPractices" class="nav1" onmouseover="displaySubMenu(this)" onmouseout="hideSubMenu(this)"><a href="https://2014.igem.org/Team:HIT-Harbin/Humanpractices">Human&nbsp;&nbsp;practices</a>
       <ul>
       <ul>
         <li>Country Profile</li>
         <li>Country Profile</li>

Revision as of 03:10, 11 October 2014

Parts

Sequence optimization was done using JCat(Java Codon Adaptation Tool)[1] developed by Andreas Grote

CODON OPTIMIZATION FOR HRPS AND HRPR GENE

Figure1: Codon Adaptation of hrpS gene in E.coli before optimization

CAI-Value of the sequence before optimization:0.2736. GC-Content of the sequence before optimization:59.17%

Figure2: Codon Adaptation of hrpS gene in E.coli after optimization

CAI-Value of the optimized sequence:0.9832 GC-Content of the optimized sequence:53.60%

Figure3: Codon Adaptation of hrpR gene in E.coli before optimization

CAI-Value of the sequence before optimization:0.2490. GC-Content of the sequence before optimization:57.70

Figure4: Codon Adaptation of hrpR gene in E.coli after optimization

CAI-Value of the optimized sequence:0.9721. GC-Content of the optimized sequence:53.69%

reference

[1] Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research 2005 Vol.33

Retrieved from "http://2014.igem.org/Team:HIT-Harbin/Judging"