Wetlab

Wetlab, wetlab and wetlab.

• 
  
  April
  
  Organization and preparation
  
  
• 
  
  May
  
  First work on BioBricks
  
  
• 
  
  June
  
  First creation of own BioBricks
  
  
• 
  
  July
  
  Improving our chip technology
  
  
• 
  
  August
  
  Adding Galectin-3 to our repertoire
  
  
• 
  
  September
  
  Getting into hot phase
  
  
• 
  
  October
  
  Finalizing our project
  
  

April

1st

Organization

where to get:

• key cards and keys for the lab
• cooled centifuge
• space in -80 °C
• bottles for 70 % ethanol
• ... (problems of a first year team ^^)

Read more about April here...

May

1st

• gel with M - full -full - REACh1 SOE3.2 - REACH2 SOE3.2 - M

  → 120 V, 30 min

  → cut out the bands

Read more about May here...

June

3rd

• transformation of 34 BioBricks

4th

• preparation of consumables:
  • fresh 50 % glycerol
  • new LB plates with cam and with kanamycin (kan)
  • 60 glas tubes
  • 2 L LB^-
  • 100 mL steril glas beads for plating

• master plates (6 clones per BioBrick) were made

Read more about June here...

July

1st

• transformation efficiency kit is broken

→ run an agarose gel with the kit plasmids to check for nuclease activity/ bands

• inventory of the -80°C freezer was done

2nd

• overnight cultures (LB) of K731520 and K1319042 for chips were inoculated

Read more about July here...

August

1st

• made electrocompetent E.coli rosetta cells.
• prepared cultures of K1319042 and K131026

2nd

• tested the OD measurement device and compared it to the spectrophotometer and the plate reader.
• tested K131026 and K1319042 for fluorescence in the plate reader
• did a heat shock transformation of I746909 into NEB TOP 10 cells
• did an electroshock transformation of pET17-Gal3 into E.coli rosetta

Read more about August here...

September

1st

• 5 ml cultures of K1319003 and K1319004
• plasmid prep

• over night cultures of K131026 in DH5α and NEB

Read more about September here...

October

1st

• Preparations for sensor-chip production on the following day (2014-10-02) was done accoringly to the sensor chip manufacturing protocol:
  • At 18:30 we prepared over-night cultures from K1319042, B0015 and K131026 by inoculating 250 ml Erlenmeyer flasks each containing 50 ml LB medium . The flasks were incubated for ~12 hours at 37 °C on a shaker.

Read more about October here...