Team:Aachen/Notebook/Wetlab2

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{{Team:Aachen/Header}}
{{Team:Aachen/Header}}
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<html><ul class="team-grid">
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<!-- team member profiles -->
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  <li><a href="https://2014.igem.org/Team:Aachen/Notebook/Wetlab/April" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> April </b>
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      <br/><br/>
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      Organization and preparation
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/3/35/Aachen_team_member_Michael_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/User:Fgohr" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> Florian Gohr </b>
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      <br/><br/>
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      <i> Molecular and Applied Biotechnology </i>
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      <br/><br/>
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      King Gibson
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/ca/Aachen_team_member_Florian_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/User:AZimmermann" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> Arne Zimmermann </b>
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      <br/><br/>
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      <i> Molecular and Applied Biotechnology M.Sc. </i>
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      <br/><br/>
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      Planning, Coordination and Fundrainsing
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/88/Aachen_team_member_Arne_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/User:Nbailly" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> Nina Bailly </b>
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      <br/><br/>
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      <i> Molecular and Applied Biotechnology </i>
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      <br/><br/>
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      Public Relations and Wiki
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/48/Aachen_team_member_Nina_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/User:VeraA" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> Vera Alexandrova </b>
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      <br/><br/>
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      <i> Biology </i>
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      <br/><br/>
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      Goddess of Plasmid Prep
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/81/Aachen_team_member_Vera_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/User:Pdemling" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> Philipp Demling </b>
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      <br/><br/>
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      <i> Molecular and Applied Biotechnology </i>
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      <br/><br/>
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      Prince of bad Puns
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      <br/><br/>
 +
      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/7/77/Aachen_team_member_Philipp_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/User:R.hanke" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> René Hanke </b>
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      <br/><br/>
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      <i> Molecular and Applied Biotechnology </i>
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      <br/><br/>
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      Commissioner for communication
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      <br/><br/>
 +
      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/b/b7/Aachen_team_member_Rene_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/User:PatrickO" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> Patrick Opdenstein </b>
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      <br/><br/>
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      <i> Molecular and Applied Biotechnology </i>
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      <br/><br/>
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      Lab servant
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      <br/><br/>
 +
      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/1/1a/Aachen_team_member_Patrick_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/User:Bpeeters" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> Björn Peeters </b>
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      <br/><br/>
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      <i> Computational Engineering Science </i>
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      <br/><br/>
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      Hardware Master
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/8d/Aachen_team_member_Bjoern_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/User:Eshani.sood" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> Eshani Sood </b>
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      <br/><br/>
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      <i> Biomedical Engineering </i>
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      <br/><br/>
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      Lab Division
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      <br/><br/>
 +
      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/52/Aachen_team_member_Eshani_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/User:Mjoppich" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> Markus Joppich </b>
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      <br/><br/>
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      <i> Computer Science </i>
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      <br/><br/>
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      Software King and Travel Assistant
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      <br/><br/>
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      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/8/83/Aachen_team_member_Markus_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/User:Ansgar" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> Ansgar Niemöller </b>
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      <br/><br/>
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      <i> Computational Engineering Science </i>
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      <br/><br/>
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      GUI Master
 +
      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/4/4e/Aachen_team_member_Ansgar_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/User:J.plum" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> Julia Plum </b>
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      <br/><br/>
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      <i> Biology and Business Administration </i>
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      <br/><br/>
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      Fundraising and Balancing
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      <br/><br/>
 +
      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/c/c9/Aachen_team_member_Julia_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/User:StefanReinhold" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> Stefan Reinhold </b>
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      <br/><br/>
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      <i> Molecular and Applied Biotechnology </i>
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      <br/><br/>
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      Treasurer
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      <br/><br/>
 +
      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/0/0c/Aachen_team_member_Stefan_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/User:Aschechtel" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> Anna Schechtel </b>
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      <br/><br/>
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      <i> Biology </i>
 +
      <br/><br/>
 +
      Chip Queen
 +
      <br/><br/>
 +
      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/a/a4/Aachen_team_member_Anna_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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  <li><a href="https://2014.igem.org/User:Suresh.sudarsan" style="color:black">
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    <div class="team-item team-info" >
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      <br/><br/>
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      <b> Dr.-Ing. Suresh Sudarsan </b>
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      <br/><br/>
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      <i>  </i>
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      <br/><br/>
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      Supervisor
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      <br/><br/>
 +
      <!-- click for more information -->
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    </div>
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    <div class="team-item team-img" style="background: url(https://static.igem.org/mediawiki/2014/5/57/Aachen_team_member_Suresh_01.jpg); norepeat scroll 0% 0% transparent; background-size:100%"> </div></a>
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  </li>
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</ul></html>
= April =
= April =
== 1st ==
== 1st ==

Revision as of 08:09, 10 October 2014


April

1st

Organization

where to get:

  • key cards and keys for the lab
  • cooled centifuge
  • space in -80 °C
  • bottles for 70 % ethanol
  • ... (problems of a first year team ^^)

Read more about April here...

May

1st

  • gel with M - full -full - REACh1 SOE3.2 - REACH2 SOE3.2 - M
    → 120 V, 30 min
    → cut out the bands

Read more about May here...

June

3rd

  • transformation of 34 BioBricks

4th

  • preparation of consumables:
    • fresh 50 % glycerol
    • new LB plates with cam and with kanamycin (kan)
    • 60 glas tubes
    • 2 L LB-
    • 100 mL steril glas beads for plating
  • master plates (6 clones per BioBrick) were made

Read more about June here...

July

1st

  • transformation efficiency kit is broken

→ run an agarose gel with the kit plasmids to check for nuclease activity/ bands

  • inventory of the -80°C freezer was done

2nd

  • overnight cultures (LB) of K731520 and K1319042 for chips were inoculated

Read more about July here...

August

1st

  • made electrocompetent E.coli rosetta cells.
  • prepared cultures of K1319042 and K131026

2nd

  • tested the OD measurement device and compared it to the spectrophotometer and the plate reader.
  • tested K131026 and K1319042 for fluorescence in the plate reader
  • did a heat shock transformation of I746909 into NEB TOP 10 cells
  • did an electroshock transformation of pET17-Gal3 into E.coli rosetta

Read more about August here...

September

1st

  • 5 ml cultures of K1319003 and K1319004
  • plasmid prep
Plasmid DNA [ng/µl]
J23101.K516032 pSB1K3 23.5
J23115.K516032 pSB1K3 20.5
J04450 pSB1A3 57.5
J04450 pSB1K1 63.5
  • over night cultures of K131026 in DH5α and NEB

Read more about September here...

October

1st

  • Preparations for sensor-chip production on the following day (2014-10-02) was done accoringly to the sensor chip manufacturing protocol:
    • At 18:30 we prepared over-night cultures from K1319042, B0015 and K131026 by inoculating 250 ml Erlenmeyer flasks each containing 50 ml LB medium . The flasks were incubated for ~12 hours at 37 °C on a shaker.

Read more about October here...


Contact Disclaimer

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