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Team:Evry/Biology/CellCharacterization/Antibiotics
From 2014.igem.org
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After having succeeded to make our bacteria grow up, we tested their resistance to different antibiotics. To know their resistance to antibiotics is very important, because it will allow us to finalize protocols of selection after tranformation. <br> | After having succeeded to make our bacteria grow up, we tested their resistance to different antibiotics. To know their resistance to antibiotics is very important, because it will allow us to finalize protocols of selection after tranformation. <br> | ||
We chose to test the most commonly used antibiotics. We included the three antibiotics used in iGEM (Kanamycin, Chloramphenicol and Ampicilin), plus the Erythromycin and the Tetracyclin. We chose the erythromycin to test a conjugaison protocol which required this antibiotic for E.Coli, and we chose to test tetracyclin because it is quite often used for inducibles systems.<br> | We chose to test the most commonly used antibiotics. We included the three antibiotics used in iGEM (Kanamycin, Chloramphenicol and Ampicilin), plus the Erythromycin and the Tetracyclin. We chose the erythromycin to test a conjugaison protocol which required this antibiotic for E.Coli, and we chose to test tetracyclin because it is quite often used for inducibles systems.<br> | ||
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<div align="center">Table 1: Concentrations tested for each antibiotic <br> | <div align="center">Table 1: Concentrations tested for each antibiotic <br> | ||
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| + | We tested several concentrations of each antibiotics (cf: Table 1), and we add a supplementary concentration for erythromycin because it is known to be not very effective on GRAM- bacteria. <br> | ||
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<img src="https://static.igem.org/mediawiki/2014/9/92/Protoantibio.jpg" alt="image not found" /> <br> | <img src="https://static.igem.org/mediawiki/2014/9/92/Protoantibio.jpg" alt="image not found" /> <br> | ||
Revision as of 13:41, 9 October 2014
Sentivity to antibiotics
After having succeeded to make our bacteria grow up, we tested their resistance to different antibiotics. To know their resistance to antibiotics is very important, because it will allow us to finalize protocols of selection after tranformation.
We chose to test the most commonly used antibiotics. We included the three antibiotics used in iGEM (Kanamycin, Chloramphenicol and Ampicilin), plus the Erythromycin and the Tetracyclin. We chose the erythromycin to test a conjugaison protocol which required this antibiotic for E.Coli, and we chose to test tetracyclin because it is quite often used for inducibles systems.
Table 1: Concentrations tested for each antibiotic
We tested several concentrations of each antibiotics (cf: Table 1), and we add a supplementary concentration for erythromycin because it is known to be not very effective on GRAM- bacteria.
