From 2014.igem.org
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| | + | <td><center><b>Thursday, May 28, 2014</b></center></td> |
| | + | <td><Prepared plasmid containing the HMF pathway; inoculated LB broth with Lambda Red Recombinase plasmid from cryogenic storage</td> |
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| | + | <td><center><b>Friday, May 29, 2014</b></center></td> |
| | + | <td>Ashlee and Emily made cryogenic storage of the Lamda Red Recombinase plasmid</td> |
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Revision as of 05:05, 19 June 2014
WELCOME TO iGEM 2014!
Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!
Click here to edit this page!
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Penn State iGEM 2014 Notebook Page
we should include weekly summaries at the head of each week (need to break up this table structure but for now it is fine) - but I think day by day lab stuff is also good (2012 igem did this)
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Biodetoxification |
Codon Optimization |
| Wednesday, May 21, 2014 |
Emily's first experience with cloning! Ashlee led Emily through several practice experiments from designs made earlier in the year: making a gel, loading samples, gel purifying DNA |
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| Thursday, May 22, 2014 |
Ashlee and Emily performed a transformation |
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| Friday, May 23, 2014 |
We picked several colonies for overnight growth to do more cloning tomorrow |
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| Saturday, May 24, 2014 |
Memorial Day Weekend? How about lab cloning weekend! Ashlee conducted plasmid preparation and digestion |
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| Monday, May 25, 2014 |
Due to a string of failed clonings with the broadhost vector pSEVA251 and the large inserts, new designs are evaluated! Instead of creating a plasmid with the HMF pathway (7.5 kb) and dCas9 system (5.5 kb), we shall add the HMF pathway and dCas9 to the P. putida genome using homologous recombination. Ordered |
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| Tuesday, May 26, 2014 |
Inoculated LB broth with ampicillin and dCas9 plasmid from cryogenic storage; inoculated Lb broth with chloramphenicol and FTV vector from Ashlee's past experiment; streaked the HMF vector on a kanamycin plate |
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| Wednesday, May 27, 2014 |
Emily and Ashlee made cryogenic storage of the dCas9 plasmid; plasmid prepared the FTV and dCas9 vectors; digested FTV vector; inoculated LB broth with a colony from the HMF plate for overnight growth and plasmid preparation tomorrow |
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| Thursday, May 28, 2014 |
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| Friday, May 29, 2014 |
Ashlee and Emily made cryogenic storage of the Lamda Red Recombinase plasmid |
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