From 2014.igem.org
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Revision as of 04:38, 19 June 2014
WELCOME TO iGEM 2014!
Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!
Click here to edit this page!
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Penn State iGEM 2014 Notebook Page
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Biodetoxification |
Codon Optimization |
| Wednesday, May 21, 2014 |
Emily's first experience with cloning! Ashlee led Emily through several practice experiments from designs made earlier in the year: making a gel, loading samples, gel purifying DNA |
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| Thursday, May 22, 2014 |
Ashlee and Emily performed a transformation |
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| Friday, May 23, 2014 |
We picked several colonies for overnight growth to do more cloning tomorrow |
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| Saturday, May 24, 2014 |
Memorial Day Weekend? How about lab cloning weekend! Ashlee conducted plasmid preparation and digestion |
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| Monday, May 25, 2014 |
Due to a string of failed clonings with the broadhost vector pSEVA251 and the large inserts, new designs are evaluated! Instead of creating a plasmid with the HMF pathway (7.5 kb) and dCas9 system (5.5 kb), we shall add the HMF pathway and dCas9 to the P. putida genome using homologous recombination. |
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