Team:Macquarie Australia

From 2014.igem.org

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The 2014 Macquarie University iGEM team is continuing the work of the 2013 team  
The 2014 Macquarie University iGEM team is continuing the work of the 2013 team  
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to synthetically construct the chlorophyll biochemical pathway in E.coli using  
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to synthetically construct the chlorophyll biochemical pathway in <i> Escherichia coli </i> using  
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synthetic Biobricks from Chlamydomonas reinhardtii. The Biobricks from 2013 have  
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synthetic Biobricks from <i> Chlamydomonas reinhardtii </i>. The Biobricks from 2013 have  
been made using Gibson Assembly. Our aim for 2014 is to improve the Biobricks using  
been made using Gibson Assembly. Our aim for 2014 is to improve the Biobricks using  
synthetic techniques, which will be assembled into three functional operons and  
synthetic techniques, which will be assembled into three functional operons and  

Revision as of 03:12, 9 October 2014

Our Project

Chlorophyll is a core component in the process of photosynthesis. As a pigment, it harvests light and plays a primary role in the excitation transfer of energy (Eichwurzel, Stiel et al. 2000), which is vital for plant reproduction and survival (Uliana, Pires et al. 2014). The chlorophyll biochemical pathway is an oxygenic photosynthetic process that oxidizes water to produce hydrogen ions. Thirteen genes govern the five-step pathway and each has a specific role.

The 2014 Macquarie University iGEM team is continuing the work of the 2013 team to synthetically construct the chlorophyll biochemical pathway in Escherichia coli using synthetic Biobricks from Chlamydomonas reinhardtii . The Biobricks from 2013 have been made using Gibson Assembly. Our aim for 2014 is to improve the Biobricks using synthetic techniques, which will be assembled into three functional operons and expressed in E.coli competent cells.