From 2014.igem.org
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| - | <h3> Protein stability analysis using Thermal and Guanadine-HCl melts </h3> | + | <h3> Protein stability analysis using Thermal and Guanidinium Hydrogen Chloride melts </h3> |
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Revision as of 01:08, 9 October 2014
Background
Our System
Method
Analysis of Test Protein Stability
Cloning in Escherichia coli
Preparation and Passaging of Saccharomyces cerevisiae
Relative Stability Analyzed via Flow Cytometry
Mutagenesis through Error Prone Polymerase Chain Reactions
Selecting Stable Variants through Fluorescence Activated Cell Sorting
Results
Protein stability analysis using Thermal and Guanidinium Hydrogen Chloride melts
Protein stability analysis using Degron Constructs and Flow Cytometry
Mutagenesis Results and Mutant Variant Analysis
Future Plans
Examination of more proteins
Submitted Parts
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